First description of Giardia duodenalis in buffalo calves (Bubalus bubalis) in southwest region of Sao Paulo State, Brazil

被引：7

作者：

Costa de Aquino, Monally Conceicao ^{[1
]}

Harvey, Tatiani Vitor ^{[2
]}

Inacio, Sandra Valeria ^{[1
]}

Nagata, Walter Bertequini ^{[1
]}

Ferrari, Elis Domingos ^{[1
]}

Miranda Oliveira, Bruno Cesar ^{[1
,3
]}

Albuquerque, George Rego ^{[2
]}

Widmer, Giovanni ^{[3
]}

Meireles, Marcelo Vasconcelos ^{[1
]}

Saraiva Bresciani, Katia Denise ^{[1
]}

机构：

[1] Univ Estadual Paulista, UNESP, Fac Med Vet, Rua Clovis Pestana 793, BR-16050680 Sao Paulo, Brazil

[2] Univ Estadual Santa Cruz UESC, Ilheus, BA, Brazil

[3] Tufts Univ, Cummings Sch Vet Med, Dept Infect Dis & Global Hlth, North Grafton, MA 01536 USA

来源：

FOOD AND WATERBORNE PARASITOLOGY | 2019年 / 16卷

基金：

巴西圣保罗研究基金会;

关键词：

Buffaloes; Genotype; Giardiasis; Multilocus genotyping; MOLECULAR CHARACTERIZATION; ASSEMBLAGE E; CRYPTOSPORIDIUM SPP; WATER-BUFFALO; DAIRY-CATTLE; IDENTIFICATION; PREVALENCE; HUMANS; INTESTINALIS; INFECTION;

D O I：

10.1016/j.fawpar.2019.e00062

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We performed molecular characterization of Giardia duodenalis in buffalo calves from the Southwest region of Sao Paulo State, Brazil. A total of 183 fecal samples of Murrah breed buffaloes up to six months of age were collected. We examined these samples by the polymerase chain reaction (PCR) targeting the small-subunit ribosomal RNA gene and positive samples were characterized using additional PCR assays targeting a portion of the beta-giardin, the glutamate dehydrogenase and the triose-phosphate isomerase genes. Based on the SSU rRNA nPCR, the presence of G. duodenalis was confirmed in 12 (6.56%) of fecal samples, of these, five, four and three samples were positive for the tpi, bg and gdh genes, respectively. Assemblage identification by sequencing was successful in 6 of 12 samples and sequence analysis showed 100% genetic similarity with G. duodenalis assemblage E. This observation represents the first detection of G. duodenalis assemblage E in buffaloes calves in Brazil. (C) 2019 Published by Elsevier Inc. on behalf of International Association of Food and Waterborne Parasitology.

引用

页数：5

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