Fluorometric label-free aptasensor for detection of the pesticideacetamiprid by using cationic carbon dots prepared with cetrimonium bromide

A fluorometricaptamer-based method is described for sensitive detection of the pesticideacetamiprid. Cationic carbon dots (cCDs) with blue fluorescence were synthesized from cetrimonium bromide (CTAB) by a hydrothermal method. In the presence of the acetamiprid aptamers with a negative charge, the aptamers bind to the surface of the cCDs due to electrostatic attraction. As a result, the fluorescence of the cCDs is quenched partially (the best measurement was done at excitation/emission wavelengths of 360/445nm). If acetamiprid is added to the above system, the aptamer binds to acetamiprid as a target with strong and specific affinity. Therefore, fluorescence increases proportionally to theacetamiprid concentrations. The aptasensor has a detection limit of 0.3nM with a dynamic range from 1.6 to 120nM which reveals that the method is sensitive in comparison to the other techniques. The selectivity of the method towards various pesticides was also studied and found to be adequate. The sensor was applied for the determination of acetamiprid in (spiked) wastewater, tap water, and tomatoes to underpin its practicability.