Inhibitors of green crab (Scylla serrata) alkaline phosphatase

被引：14

作者：

Chen, QX

Zhou, HM ^{[1
]}

机构：

[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China

[2] Acad Sinica, Inst Biophys, Natl Lab, Beijing 100101, Peoples R China

来源：

JOURNAL OF ENZYME INHIBITION | 1999年 / 14卷 / 03期

关键词：

alkaline phosphatase; green crab; enzymatic/catalytic mechanism; inactivation; inhibition;

D O I：

10.3109/14756369909030320

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Green crab (Scylla serrata) alkaline phosphatase (EC 3.1.3.1) is a metalloenzyme which catalyzes the nonspecific hydrolysis of phosphate monoesters. The effects of several inhibitors on its activity have been studied. The results show that Na2HPO4, Na2HAsO4 and Na2WO4 are competitive inhibitors, L-phenylalanine is an uncompetitive inhibitor, while L-cysteine is a mixed-type inhibitor. The equilibrium constants were determined for inhibitor binding. The order of potency was Na2HPO4 (K-1 = 0.95 mM) > Na2AsO4 (1.10 mM) > Na2WO4(1.55 mM).

引用

页码：251 / 257

页数：7

共 11 条

[1] Kinetics of inactivation of green crab (Scylla Serrata) alkaline phosphatase during removal of zinc ions by ethylenediaminetetraacetic acid disodium [J].

Chen, QX ;

Zhang, W ;

Wang, HR ;

Zhou, HM .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1996, 19 (04) :257-261

[2] Kinetics of inhibition of alkaline phosphatase from green crab (Scylla serrata) by N-bromosnccinimide [J].

Chen, QX ;

Zhang, W ;

Zheng, WZ ;

Zhao, H ;

Yan, SX ;

Wang, HR ;

Zhou, HM .

JOURNAL OF PROTEIN CHEMISTRY, 1996, 15 (04) :345-350

[3]

COLEMAN JE, 1992, ANNU REV BIOPH BIOM, V21, P441, DOI 10.1146/annurev.biophys.21.1.441

[4]

CYBORON GW, 1981, J BIOL CHEM, V256, P7262

[5]

Fernley H., 1971, The Enzymes, V4, P417

[6]

HARRIS H, 1989, CLIN CHIM ACTA, V186, P133

[7] REACTION-MECHANISM OF ALKALINE-PHOSPHATASE BASED ON CRYSTAL-STRUCTURES - 2-METAL ION CATALYSIS [J].

KIM, EE ;

WYCKOFF, HW .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 218 (02) :449-464

[8]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[9]

McComb R. B., 1979, ALKALINE PHOSPHATASE

[10]

Xie WZ, 1996, BIOCHEM MOL BIOL INT, V40, P981

← 1 2 →