Germline Stem Cells Drive Ovary Regeneration in Zebrafish

被引：63
作者：
Cao, Zigang [1 ,2 ]
Mao, Xiaoyu [1 ]
Luo, Lingfei [1 ]
机构：
[1] Southwest Univ, Lab Mol Dev Biol, Minist Educ, Key Lab Freshwater Fish Reprod & Dev,Sch Life Sci, Chongqing 400715, Peoples R China
[2] Jinggangshan Univ, Coll Life Sci, Jiangxi Key Lab Organ Dev Biol, Jian 343009, Jiangxi, Peoples R China
来源：
CELL REPORTS | 2019年 / 26卷 / 07期
基金：
中国国家自然科学基金;
关键词：
EXPRESSION; MAINTAIN; RNA; TRANSGENE; COMPONENT; PROMOTER; ABLATION; ADHESION; OOCYTES; PROTEIN;
D O I：
10.1016/j.celrep.2019.01.061
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that nanos2, a conserved vertebrate GSC marker, is required for maintaining GSCs in zebrafish. We applied genetic ablation and tissue resection techniques to delineate the function of GSCs in zebrafish ovary regeneration. After GSC ablation, ovaries fail to regenerate and are converted to sterile testes. Amputated ovarian tissues completely regenerate as a result of the proliferation of residual GSCs, but nanos2 mutant ovaries fail to regenerate after amputation due to a lack of GSCs. The repression of Wnt signaling leads to reduced numbers of GSCs and delayed ovary regeneration. Our results provide insight into the key role of GSCs in driving ovary regeneration.
引用
收藏
页码：1709 / +
页数：12
相关论文
共 39 条
[11]   Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish [J].
Hruscha, Alexander ;
Krawitz, Peter ;
Rechenberg, Alexandra ;
Heinrich, Verena ;
Hecht, Jochen ;
Haass, Christian ;
Schmid, Bettina .
DEVELOPMENT, 2013, 140 (24) :4982-4987
[12]   Nitroreductase-mediated Gonadal Dysgenesis for Infertility Control of Genetically Modified Zebrafish [J].
Hu, Shao-Yang ;
Lin, Pei-Yu ;
Liao, Chia-Hsuan ;
Gong, Hong-Yi ;
Lin, Gen-Hwa ;
Kawakami, Koichi ;
Wu, Jen-Leih .
MARINE BIOTECHNOLOGY, 2010, 12 (05) :569-578
[13]   Germline stem cells and follicular renewal in the postnatal mammalian ovary [J].
Johnson, J ;
Canning, J ;
Kaneko, T ;
Pru, JK ;
Tilly, JL .
NATURE, 2004, 428 (6979) :145-150
[14]   Wnt/β-catenin signaling regulates vertebrate limb regeneration [J].
Kawakami, Yasuhiko ;
Esteban, Concepcion Rodriguez ;
Raya, Marina ;
Kawakami, Hiroko ;
Marti, Merce ;
Dubova, Ilir ;
Izpisua Belmonte, Juan Carlos .
GENES & DEVELOPMENT, 2006, 20 (23) :3232-3237
[15]   Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification [J].
Knaut, H ;
Pelegri, F ;
Bohmann, K ;
Schwarz, H ;
Nüsslein-Volhard, C .
JOURNAL OF CELL BIOLOGY, 2000, 149 (04) :875-888
[16]   Expression of a vas::EGFP transgene in primordial germ cells of the zebrafish [J].
Krovel, AV ;
Olsen, LC .
MECHANISMS OF DEVELOPMENT, 2002, 116 (1-2) :141-150
[17]   THE GENE ENCODING A MAJOR COMPONENT OF THE LATERAL ELEMENTS OF SYNAPTONEMAL COMPLEXES OF THE RAT IS RELATED TO X-LINKED LYMPHOCYTE-REGULATED GENES [J].
LAMMERS, JHM ;
OFFENBERG, HH ;
VANAALDEREN, M ;
VINK, ACG ;
DIETRICH, AJJ ;
HEYTING, C .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (02) :1137-1146
[18]   The ziwi Promoter Drives Germ line-Specific Gene Expression in Zebrafish [J].
Leu, David H. ;
Draper, Bruce W. .
DEVELOPMENTAL DYNAMICS, 2010, 239 (10) :2714-2721
[19]   Macrophages Mediate the Repair of Brain Vascular Rupture through Direct Physical Adhesion and Mechanical Traction [J].
Liu, Chi ;
Wu, Chuan ;
Yang, Qifen ;
Gao, Jing ;
Li, Li ;
Yang, Deqin ;
Luo, Lingfei .
IMMUNITY, 2016, 44 (05) :1162-1176
[20]   Tandem-repeated zebrafish zp3 genes possess oocyte-specific promoters and are insensitive to estrogen induction [J].
Liu, Xingjun ;
Wang, Hai ;
Gong, Zhiyuan .
BIOLOGY OF REPRODUCTION, 2006, 74 (06) :1016-1025
1234