Germline Stem Cells Drive Ovary Regeneration in Zebrafish

被引：63
作者：
Cao, Zigang [1 ,2 ]
Mao, Xiaoyu [1 ]
Luo, Lingfei [1 ]
机构：
[1] Southwest Univ, Lab Mol Dev Biol, Minist Educ, Key Lab Freshwater Fish Reprod & Dev,Sch Life Sci, Chongqing 400715, Peoples R China
[2] Jinggangshan Univ, Coll Life Sci, Jiangxi Key Lab Organ Dev Biol, Jian 343009, Jiangxi, Peoples R China
来源：
CELL REPORTS | 2019年 / 26卷 / 07期
基金：
中国国家自然科学基金;
关键词：
EXPRESSION; MAINTAIN; RNA; TRANSGENE; COMPONENT; PROMOTER; ABLATION; ADHESION; OOCYTES; PROTEIN;
D O I：
10.1016/j.celrep.2019.01.061
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that nanos2, a conserved vertebrate GSC marker, is required for maintaining GSCs in zebrafish. We applied genetic ablation and tissue resection techniques to delineate the function of GSCs in zebrafish ovary regeneration. After GSC ablation, ovaries fail to regenerate and are converted to sterile testes. Amputated ovarian tissues completely regenerate as a result of the proliferation of residual GSCs, but nanos2 mutant ovaries fail to regenerate after amputation due to a lack of GSCs. The repression of Wnt signaling leads to reduced numbers of GSCs and delayed ovary regeneration. Our results provide insight into the key role of GSCs in driving ovary regeneration.
引用
收藏
页码：1709 / +
页数：12
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