Enhancement of liposome mediated gene transfer by adding cholesterol and cholesterol modulating drugs

被引：17

作者：

Bae, Yun-Ui ^{[1
]}

Huh, Jae-Wan ^{[2
]}

Kim, Bieong-Kil ^{[3
]}

Park, Hyeon Young ^{[1
]}

Seu, Young-Bae ^{[3
]}

Doh, Kyung-Oh ^{[1
]}

机构：

[1] Yeungnam Univ, Dept Physiol, Coll Med, Daegu 42415, South Korea

[2] Univ Ulsan, Coll Med, Dept Biochem & Mol Biol, Seoul 05505, South Korea

[3] Kyungpook Natl Univ, Sch Life Sci & Biotechnol, Daegu 41566, South Korea

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2016年 / 1858卷 / 12期

关键词：

Liposomes; Transfection; Cholesterol; Gene therapy; DNA COMPLEXES; INTRACELLULAR TRAFFICKING; CATIONIC LIPIDS; CELL-LINE; DELIVERY; EFFICIENCY; PATHWAYS; SERUM; INHIBITION; AUTOPHAGY;

D O I：

10.1016/j.bbamem.2016.09.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol is an important cell membrane component and has been used as co-lipid for cationic liposome to enhance gene delivery. However, the role of cholesterol in transfection efficiency has not been fully understood. In this study, transfection efficiency of liposome was measured after cholesterol was added to the cell culture medium. As a result, addition of cholesterol increased transfection efficiency of several liposomes consisting of different lipid components in various cells (AGS, CHO, COS7 and, MCF7). Furthermore, treatment of cells with cholesterol modulating drugs, imipramine and U18666A, also increased transfection efficiency of liposomes. To elucidate the role of added cholesterol in gene transfer, endocytotic mechanism was studied and also revealed that adding cholesterol in culture media induced participation of caveolae-mediated endocytosis and micropinocytosis in CHO cell. Therefore, the results of this work suggest that modulation of intracellular cholesterol can be an important method to enhance gene delivery. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：3017 / 3023

页数：7

共 36 条

[1] Endocytic Pathway and Resistance to Cholesterol Depletion of Cholesterol Derived Cationic Lipids for Gene Delivery [J].

Bae, Yun-Ui ;

Kim, Bieong-Kil ;

Park, Jong-Won ;

Seu, Young-Bae ;

Doh, Kyung-Oh .

MOLECULAR PHARMACEUTICS, 2012, 9 (12) :3579-3585

[2] Cardiac Stim1 Silencing Impairs Adaptive Hypertrophy and Promotes Heart Failure Through Inactivation of mTORC2/Akt Signaling [J].

Benard, Ludovic ;

Oh, Jae Gyun ;

Cacheux, Marine ;

Lee, Ahyoung ;

Nonnenmacher, Mathieu ;

Matasic, Daniel S. ;

Kohlbrenner, Erik ;

Kho, Changwon ;

Pavoine, Catherine ;

Hajjar, Roger J. ;

Hulot, Jean-Sebastien .

CIRCULATION, 2016, 133 (15) :1458-1471

[3] Cholesterol-Dependent Macropinocytosis and Endosomal Escape Control the Transfection Efficiency of Lipoplexes in CHO Living Cells [J].

Cardarelli, Francesco ;

Pozzi, Daniela ;

Bifone, Angelo ;

Marchini, Cristina ;

Caracciolo, Giulio .

MOLECULAR PHARMACEUTICS, 2012, 9 (02) :334-340

[4] Cholesterol Synthesis Inhibitor U18666A and the Role of Sterol Metabolism and Trafficking in Numerous Pathophysiological Processes [J].

Cenedella, Richard J. .

LIPIDS, 2009, 44 (06) :477-487

[5] Regulated portals of entry into the cell [J].

Conner, SD ;

Schmid, SL .

NATURE, 2003, 422 (6927) :37-44

[6] Inclusion of cholesterol in DOTAP transfection complexes increases the delivery of DNA to cells in vitro in the presence of serum [J].

Crook, K ;

Stevenson, BJ ;

Dubouchet, M ;

Porteous, DJ .

GENE THERAPY, 1998, 5 (01) :137-143

[7] Cell line-dependent internalization pathways and intracellular trafficking determine transfection efficiency of nanoparticle vectors [J].

Douglas, Kimberly L. ;

Piccirillo, Ciriaco A. ;

Tabrizian, Maryam .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2008, 68 (03) :676-687

[8] An overview of current delivery systems in cancer gene therapy [J].

El-Aneed, A .

JOURNAL OF CONTROLLED RELEASE, 2004, 94 (01) :1-14

[9] Niemann-Pick C1 Affects the Gene Delivery Efficacy of Degradable Polymeric Nanoparticles [J].

Eltoukhy, Ahmed A. ;

Sahay, Gaurav ;

Cunningham, James M. ;

Anderson, Daniel G. .

ACS NANO, 2014, 8 (08) :7905-7913

[10] Cationic lipid-DNA complexes for gene therapy: Understanding the relationship between complex structure and gene delivery pathways at the molecular level [J].

Ewert, K ;

Slack, NL ;

Ahmad, A ;

Evans, HM ;

Lin, AJ ;

Samuel, CE ;

Safinya, CR .

CURRENT MEDICINAL CHEMISTRY, 2004, 11 (02) :133-149

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