lncRNA FGD5-AS1 Regulates Bone Marrow Stem Cell Proliferation and Apoptosis by Affecting miR-296-5p/STAT3 Axis in Steroid-Induced Osteonecrosis of the Femoral Head

被引：3
作者：
Wu, Yadi [1 ]
Fang, Lun [1 ]
Gao, Yong [2 ]
Zhao, Zhiqiu [1 ]
Zhou, Lu [3 ]
Zhang, Gang [2 ]
机构：
[1] Shandong First Med Univ, Shandong Acad Med Sci, Tai An 271016, Shandong, Peoples R China
[2] Shandong First Med Univ, Dept Orthoped, Affiliated Hosp 2, Tai An 271000, Shandong, Peoples R China
[3] Shandong First Med Univ, Shandong Acad Med Sci, Inst Sports Med, Tai An 271016, Shandong, Peoples R China
来源：
JOURNAL OF HEALTHCARE ENGINEERING | 2022年 / 2022卷
关键词：
NONTRAUMATIC OSTEONECROSIS; DIFFERENTIATION; CANCER; PROGRESSION;
D O I：
10.1155/2022/9364467
中图分类号：
R19 [保健组织与事业（卫生事业管理）];
学科分类号：
摘要：
Background. Osteonecrosis of the femoral head (ONFH) is a common hip joint disease, which is more harmful and seriously affects the lives of patients. This study aims to clarify the regulatory mechanism of lncRNA FGD5-AS1 in ONFH. Methods. The expression of the protein and mRNA was detected by RT-qPCR and Western blot assay. The regulatory mechanism of lncRNA FGD5-AS1 was detected by the dual-luciferase reporter assay, CCK-8 assay, and flow cytometry assay. Results. Dex can inhibit cell proliferation and differentiation and induce apoptosis in hBMSCs in a dose-dependent manner. Overexpression of lncRNA FGD5-AS1 promoted cell proliferation and restrained apoptosis in Dex-treated hBMSCs. In addition, lncRNA FGD5-AS1 acts as a sponge for miR-296-5p. Also, miR-296-5p directly targets STAT3. More importantly, miR-296-5p and STAT3 can affect the function of lncRNA FGD5-AS1 in Dex-treated hBMSCs. Conclusion. lncRNA FGD5-AS1 promotes cell proliferation and inhibits apoptosis in steroid-induced ONFH through acting as a sponge for miR-296-5p and upregulation of STAT3.
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页数：9
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  • [1] Circ-E2F3 promotes cervical cancer progression by inhibiting microRNA-296-5p and increasing STAT3 nuclear translocation
    Cao, Xiangke
    Ma, Qinghua
    Wang, Bin
    Qian, Qingqiang
    Xi, Yinan
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 2022, 1507 (01) : 84 - 98
  • [2] Astragaloside IV Improves Tibial Defect in Rats and Promotes Proliferation and Osteogenic Differentiation of hBMSCs through MiR-1 24-3p.1/STAT3 Axis
    Cao, Yujing
    Lv, Qiuxia
    Li, Yang
    [J]. JOURNAL OF NATURAL PRODUCTS, 2021, 84 (02): : 287 - 297
  • [3] LncRNA AWPPH participates in the development of non-traumatic osteonecrosis of femoral head by upregulating Runx2
    Chen, Xiantao
    Li, Jing
    Liang, Dawei
    Zhang, Leilei
    Wang, Qingfeng
    [J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2020, 19 (01) : 153 - 159
  • [4] Pseudogene HSPB1P1 contributes to renal cell carcinoma proliferation and metastasis by targeting miR-296-5p to regulate HMGA1 expression
    Chen, Zerong
    Wang, Ziming
    Chen, Zhuangfei
    Fu, Fangxiang
    Huang, Xiaomin
    Huang, Zehai
    [J]. CELL BIOLOGY INTERNATIONAL, 2021, 45 (12) : 2479 - 2489
  • [5] Circ_0076305 regulates cisplatin resistance of non-small cell lung cancer via positively modulating STAT3 by sponging miR-296-5p
    Dong, Yu
    Xu, Tao
    Zhong, Shouping
    Wang, Bo
    Zhang, Huimin
    Wang, Xin
    Wang, Peng
    Li, Guangshun
    Yang, Shuanying
    [J]. LIFE SCIENCES, 2019, 239
  • [6] LncRNA NORAD promotes bone marrow stem cell differentiation and proliferation by targeting miR-26a-5p in steroid-induced osteonecrosis of the femoral head
    Fu, Dapeng
    Yang, Sheng
    Lu, Jianmin
    Lian, Haoyi
    Qin, Kairong
    [J]. STEM CELL RESEARCH & THERAPY, 2021, 12 (01)
  • [7] Non traumatic osteonecrosis of the femoral head is associated with low bone mass
    Gangji, Valerie
    Soyfoo, Muhammad S.
    Heuschling, Audrey
    Afzali, Violaine
    Moreno-Reyes, Rodrigo
    Rasschaert, Joanne
    Gillet, Celine
    Fils, Jean-Francois
    Hauzeur, Jean-Philippe
    [J]. BONE, 2018, 107 : 88 - 92
  • [8] Non-coding RNA Identification in Osteonecrosis of the Femoral Head Using Competitive Endogenous RNA Network Analysis
    Han, Ning
    Li, Zengchun
    [J]. ORTHOPAEDIC SURGERY, 2021, 13 (03) : 1067 - 1076
  • [9] Aloperine Inhibits Proliferation and Promotes Apoptosis in Colorectal Cancer Cells by Regulating the circNSUN2/miR-296-5p/STAT3 Pathway
    Han, Wei
    Kong, Desong
    Lu, Qin
    Zhang, Wei
    Fan, Zhimin
    [J]. DRUG DESIGN DEVELOPMENT AND THERAPY, 2021, 15 : 857 - 870
  • [10] Upregulation of Long Noncoding RNA FGD5-AS1 Ameliorates Myocardial Ischemia/Reperfusion Injury via MicroRNA-106a-5p and MicroRNA-106b-5p
    Hao, Lin
    Wang, Juan
    Bi, Shao-Jie
    Cheng, Chao
    [J]. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2021, 78 (01) : 45 - 54
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