Investigation of metallo-beta-lactamase producing strains of Pseudomonas aeruginosa and Acinetobacter baumannii by E-test, disk synergy and PCR

被引：18

作者：

Aktas, Zerrin ^{[1
]}

Kayacan, Cigdem Bal ^{[1
]}

机构：

[1] Istanbul Univ, Fac Med, Dept Microbiol & Clin Microbiol, TR-34300 Istanbul, Turkey

来源：

SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES | 2008年 / 40卷 / 04期

关键词：

D O I：

10.1080/00365540701704698

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Carbapenem non-susceptible Pseudomonas aeruginosa and Acinetobacter baumannii strains were tested for the presence of metallo-beta-lactamases (MBLs) by EDTA-synergy screening. Imipenem hydrolysis was investigated by a bioassay and IMP-/VIM-encoding genes by PCR. No blaIMP/VIM related genes or imipenemase activity were detected although E-test found all strains as MBL-positive. Disk synergy tests with 0.5M EDTA determined 63.6-100%, while those with 0.1M EDTA detected 0-7.7% of isolates as MBL producers. Most strains were susceptible to EDTA. In conclusion, for MBL-screening purposes, EDTA-synergy results change with molarity of EDTA, but even if some false positives are encountered, 0.1M EDTA seems to be acceptable.

引用

页码：320 / 325

页数：6

共 24 条

[1] First IMP-1-producing Klebsiella pneumoniae isolate in Turkey [J].

Aktas, Z. ;

Bal, C. ;

Midilli, K. ;

Poirel, L. ;

Nordmann, P. .

CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 (07) :695-696

[2]

[Anonymous], [No title captured]

[3]

Arakawa Y, 2000, J CLIN MICROBIOL, V38, P40

[4] Effect of permeabilizers on antibiotic sensitivity of Pseudomonas aeruginosa [J].

Ayres, HM ;

Furr, JR ;

Russell, AD .

LETTERS IN APPLIED MICROBIOLOGY, 1999, 28 (01) :13-16

[5] Detection of VIM-5 metallo-β-lactamase in a Pseudomonas aeruginosa clinical isolate from Turkey [J].

Bahar, G ;

Mazzariol, A ;

Koncan, R ;

Mert, A ;

Fontana, R ;

Rossolini, GM ;

Cornaglia, G .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2004, 54 (01) :282-283

[6] A novel type of AmpC β-lactamase, ACC-1, produced by a Klebsiella pneumoniae strain causing nosocomial pneumonia [J].

Bauernfeind, A ;

Schneider, I ;

Jungwirth, R ;

Sahly, H ;

Ullmann, U .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (08) :1924-1931

[7] A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE [J].

BUSH, K ;

JACOBY, GA ;

MEDEIROS, AA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1211-1233

[8] EDTA susceptibility leading to false detection of metallo-β-lactamase in Pseudomonas aeruginosa by Etest and an imipenem-EDTA disk method [J].

Chu, YW ;

Cheung, TKM ;

Ngan, JYW ;

Kam, KM .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2005, 26 (04) :340-341

[9] IMP-4, a novel metallo-β-lactamase from nosocomial Acinetobacter spp. collected in Hong Kong between 1994 and 1998 [J].

Chu, YW ;

Afzal-Shah, M ;

Houang, ETS ;

Palepou, MFI ;

Lyon, DJ ;

Woodford, N ;

Livermore, DM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (03) :710-714

[10] Outbreak of carbapenem-resistant Pseudomonas aeruginosa producing VIM-8, a novel metallo-β-lactamase, in a tertiary care center in Cali, Colombia [J].

Crespo, MP ;

Woodford, N ;

Sinclair, A ;

Kaufmann, ME ;

Turton, J ;

Glover, J ;

Velez, JD ;

Castañeda, CR ;

Recalde, M ;

Livermore, DM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (11) :5094-5101

← 1 2 3 →