Anti-myelin associated glycoprotein antibodies recognize HNK-1 epitope on CNS

被引：8

作者：

Mata, Sabrina ^{[1
]}

Ambrosini, Stefano ^{[1
]}

Mello, Tommaso ^{[2
]}

Lolli, Francesco ^{[1
]}

Minciacchi, Diego ^{[3
]}

机构：

[1] Univ Florence, Dipartimento Sci Neurol & Psichiatr, I-50134 Florence, Italy

[2] Univ Florence, Dept Clin Pathophysiol, Gastroenterol Unit, I-50134 Florence, Italy

[3] Univ Florence, Dept Physiol Sci, I-50134 Florence, Italy

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2011年 / 236卷 / 1-2期

关键词：

Myelin-associated glycoprotein (MAG); Rat; Demyelinating polyneuropathy; Indirect immunofluorescence; MYELIN-ASSOCIATED GLYCOPROTEIN; ANTI-MAG ANTIBODIES; NERVOUS-SYSTEM; L2/HNK-1; CARBOHYDRATE; ADHESION MOLECULES; MONOCLONAL IGM; CA2; SUBFIELD; POLYNEUROPATHY; NEUROPATHY; EXPRESSION;

D O I：

10.1016/j.jneuroim.2011.05.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Antibodies to myelin-associated glycoprotein (MAC) are associated with demyelinating polyneuropathy and are specific for the HNK-1 epitope. To test if anti-MAC IgM recognize HNK-1 on CNS, sera from 20 patients and 238 controls were tested on rat slices by indirect immunofluorescence (IF). IgM from anti-MAC positive patients, but not from control sera, stained rat brain with perineuronal or neuropil pattern, depending on the CNS region. IIF titers significantly correlated with ELISA anti-MAC titers. The staining of patients' sera were inhibited by mouse anti-HNK-1 monoclonal antibody. Our results demonstrate that anti-MAG IgM recognizes HNK-1 outside the peripheral nerve myelin carriers. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：99 / 105

页数：7

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