Optimization of enzymatic hydrolysis conditions of Golden Apple snail (Pomacea canaliculata) protein by Alcalase

The objective of this study is to establish conditions that allow optimal yield and antioxidant activity for Golden Apple Snail (GAS) (Panacea canaliculata) protein hy droly sate by employing response surface methodology (RSM). A three level, face-centered, central composite design (CCD) was adapted to assess the effects of temperature (45-65 degrees C); pH (8-10): the ratio of enzyme to substrate (2-4%); and hydrolysis time (60-180 nun). The antioxidative activity of the hydrolysate obtained under optimized conditions was then evaluated via the following metrics: hydroxyl radical scavenging, reducing power, and chelating effects on ferrous ion. Established optimal conditions for the enzymatic protein hydrolysis of GAS were a temperature of 45 degrees C, a pH of 10, an enzyme concentration of 2%, and hydrolysis time of 159 minutes. The optimized GAS protein hydrolysate produced an experimental yield of 9.72% and antioxidant activity of 73.54% slightly less than the predicted yield of 11.36% and antioxidant activity of 78.88%. The optimized GAS protein hydrolysate formed demonstrated both higher chelating effects and hydroxyl scavenging activity but had lower reducing power. These results suggest that GAS protein hydrolysate holds potential as a natural antioxidant for use in food processing. (C) All Rights Reserved