SRG3, a core component of mouse SWI/SNF complex, is essential for extra-embryonic vascular development

被引：33
作者：
Han, Daehee [1 ,2 ]
Jeon, Shin [1 ,2 ]
Sohn, Dong Hyun [1 ,2 ]
Lee, Changjin [1 ,2 ]
Ahn, Sangil [3 ]
Kim, Won Kyu [3 ]
Chung, Heekyoung [4 ]
Seong, Rho Hyun [1 ,2 ]
机构：
[1] Seoul Natl Univ, Inst Mol Biol & Genet, Dept Biol Sci, Seoul 151742, South Korea
[2] Seoul Natl Univ, Res Ctr Funct Cellulom, Seoul 151742, South Korea
[3] Hanyang Univ, Coll Med, Dept Anat & Cell Biol, Seoul 133791, South Korea
[4] Hanyang Univ, Coll Med, Dept Pathol, Seoul 133791, South Korea
来源：
DEVELOPMENTAL BIOLOGY | 2008年 / 315卷 / 01期
关键词：
SRG3; SWI/SNF complex; transgene-rescue; angiogenesis; yolk sac; blood vessel; visceral endoderm;
D O I：
10.1016/j.ydbio.2007.12.024
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The SWI/SNF chromatin-remodeling complex functions as a transcriptional regulator and plays a significant role in cell proliferation, differentiation and embryonic development. SRG3, a homologue of human BAF155, is a core component of the mouse SWI/SNF chromatin remodeling complex. Mutant mice deficient in Srg3 expression are peri-implantation lethal. To investigate the role of SRG3 in the post-implantation stage, we generated SRG3 transgene-rescued (Srg3-/-Tg+) embryos by inducing exogenous gene expression. These Srg3-/-Tg+ embryos overcame early embryonic lethality and extended the life span until mid-gestation. However, the embryos displayed significant defects in blood vessel formation and fetal circulation within the yolk sac around embryonic day 10.5, which led to developmental retardation and death. We found that SRG3 expression was absent in the visceral endoderm of Srg3-/-Tg+ yolk sacs, while SRG3 was normally expressed in wild-type embryos. In addition, expression of angiogenesis-related genes, including Angiopoietin1, Tie2, EphrinB2, Ihh and Notch1, was markedly reduced in Srg3-/-Tg+ yolk sacs. During normal angiogenesis, maturation of the visceral endoderm development is observed in the yolk sac. However, in Srg3-/-Tg+ yolk sacs, the visceral endoderm did not develop normally. Our results indicate that SRG3 is required for angiogenesis and visceral endoderm development in the yolk sac. (C) 2007 Elsevier Inc. All rights reserved.
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收藏
页码：136 / 146
页数：11
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