Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease

被引：24

作者：

Alonso-Padilla, Julio ^{[1
]}

Jimenez de Oya, Nereida ^{[1
]}

Blazquez, Ana-Belen ^{[1
]}

Escribano-Romero, Estela ^{[1
]}

Escribano, Jose M. ^{[1
]}

Saiz, Juan-Carlos ^{[1
]}

机构：

[1] Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Dept Biotechnol, Madrid 28040, Spain

来源：

VACCINE | 2011年 / 29卷 / 09期

关键词：

Vaccine; Passive immune transfer; Insect biofactories; HEPATITIS-E VIRUS; CD8(+) T-CELLS; ENCEPHALITIS-VIRUS; DISSEMINATED INFECTION; VACCINE DEVELOPMENT; ANTIBODIES; IMMUNIZATION; DIAGNOSIS; IMMUNITY; BACULOVIRUS;

D O I：

10.1016/j.vaccine.2010.12.081

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, West Nile virus (WNV) envelope (rE) protein and its domain III (rDIII) were efficiently expressed in a cost-effective system based on insect larvae as non-fermentative living biofactories. Mice immunized with the partially purified rE or rDIII elicited high antibodies titers that neutralized viral infectivity in cell culture and in suckling mice. All vaccinated animals were fully protected when challenged with neurovirulent WNV NY99. Passive transfer of protective antibodies from immunized mothers to their offspring occurred both by transplacental and lactation routes. These results indicate that the insect-derived antigens tested may constitute potential vaccine candidates to be further evaluated. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1830 / 1835

页数：6

共 45 条

[1] Evaluation of an enzyme-linked immunosorbent assay for detection of West Nile virus infection based on a recombinant envelope protein produced in Trichoplusia ni larvae [J].

Alonso-Padilla, Julio ;

Jimenez de Oya, Nereida ;

Blazquez, Ana-Belen ;

Loza-Rubio, Elizabeth ;

Escribano, Jose M. ;

Saiz, Juan-Carlos ;

Escribano-Romero, Estela .

JOURNAL OF VIROLOGICAL METHODS, 2010, 166 (1-2) :37-41

[2] Isolation of West Nile virus from mosquitoes, crows, and a Cooper's hawk in Connecticut [J].

Anderson, JF ;

Andreadis, TG ;

Vossbrinck, CR ;

Tirrell, S ;

Wakem, EM ;

French, RA ;

Garmendia, AE ;

Van Kruiningen, HJ .

SCIENCE, 1999, 286 (5448) :2331-2333

[3]

Baitchman EJ, 2007, J ZOO WILDLIFE MED, V38, P337, DOI 10.1638/1042-7260(2007)038[0337:PTOMAT]2.0.CO

[4]

[5] Flavivirus DNA vaccine with a kick [J].

Barrett, Alan D. T. .

NATURE BIOTECHNOLOGY, 2008, 26 (05) :525-526

[6]

BEATY BJ, 1989, ARBOVIRUSES, P795

[7] West Nile virus (WNV) transmission routes in the murine model: Intrauterine, by breastfeeding and after cannibal ingestion [J].

Blazquez, Ana-Belen ;

Saiz, Juan-Carlos .

VIRUS RESEARCH, 2010, 151 (02) :240-243

[8]

Blitvich Bradley J., 2008, Animal Health Research Reviews, V9, P71, DOI 10.1017/S1466252307001430

[9] A recombinant West Nile virus envelope protein vaccine candidate produced in Spodoptera frugiperda expresSF plus cells [J].

Bonafe, Nathalie ;

Rininger, Joseph A. ;

Chubet, Richard G. ;

Foellmer, Harald G. ;

Fader, Stacey ;

Anderson, John F. ;

Bushmich, Sandra L. ;

Anthony, Karen ;

Ledizet, Michel ;

Fikrig, Erol ;

Koski, Raymond A. ;

Kaplan, Paul .

VACCINE, 2009, 27 (02) :213-222

[10] The molecular biology of West Nile virus: A new invader of the Western hemisphere [J].

Brinton, MA .

ANNUAL REVIEW OF MICROBIOLOGY, 2002, 56 :371-402

← 1 2 3 4 5 →