Anti-Inflammatory and Healing Activity of the Hydroalcoholic Fruit Extract of Solanum diploconos (Mart.) Bohs

被引：10

作者：

Benvenutti, Larissa ^{[1
]}

Nunes, Roberta ^{[1
]}

Venturi, Ivonilce ^{[1
]}

Ramos, Silvia Aparecida ^{[1
]}

Broering, Milena Fronza ^{[1
]}

Goldoni, Fernanda Capitanio ^{[2
]}

Pavan, Sarah Eskelsen ^{[2
]}

Pastor, Maria Veronica Davila ^{[2
]}

Malheiros, Angela ^{[1
,2
]}

Quintao, Nara Lins Meira ^{[1
,2
]}

Fernandes, Elizabeth Soares ^{[3
,4
]}

Santin, Jose Roberto ^{[1
,2
]}

机构：

[1] Univ Vale Itajai, Postgrad Program Pharmaceut Sci, Rua Uruguai,458, BR-88302202 Itajai, SC, Brazil

[2] Univ Vale Itajai, Sch Hlth Sci, Biomed Course, Rua Uruguai,458, BR-88302202 Itajai, SC, Brazil

[3] Programa Posgrad Biotecnol Aplicada, Crianca Adolescente, Fac Pequeno Principe, Av Iguacu,333, BR-80230020 Curitiba, PR, Brazil

[4] Inst Pesquisa Pele Pequeno Principe, Av Silva Jardim 1632, BR-80250060 Curitiba, PR, Brazil

来源：

JOURNAL OF IMMUNOLOGY RESEARCH | 2021年 / 2021卷

关键词：

ACID PHENETHYL ESTER; NF-KAPPA-B; IN-VITRO; EXPRESSION; ANTIOXIDANT; MACROPHAGES; RESOLUTION; MIGRATION; PATHWAYS; CAPACITY;

D O I：

10.1155/2021/9957451

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. Solanum diploconos (Mart.) Bohs is a native Brazilian plant belonging to the Solanaceae family, popularly known as "tomatinho do mato" and poorly investigated. Herein, we presented for the first time evidence for the anti-inflammatory and wound healing activities of S. diploconos fruit hydroalcoholic extract. Material and Methods. In vitro fMLP-induced chemotaxis, LPS-induced inflammatory mediator levels (cytokines by ELISA and NO release by Griess reaction), and adhesion molecule expression (CD62L, CD49d, and CD18, by flow-cytometry) were assessed in neutrophils treated with different concentrations of the extract. Inflammation resolution was measured by the efferocytosis assay and the healing activity by in vivo and in vitro assays. The air pouch model of carrageenan-induced inflammation in Swiss mice was used to investigate the in vivo anti-inflammatory effects of the extract. Leukocyte influx (by optical microscopy) and cytokine release were quantified in the pouch exudates. Additionally, the acute and subacute toxic and genotoxic effects of the extract were evaluated. Results. In vitro, the extract impaired neutrophil chemotaxis and its ability to produce and/or release cytokines (TNF alpha, IL-1 beta, and IL-6) and NO upon LPS stimuli (p<0.01). LPS-treated neutrophils incubated with the extract presented increased CD62L expression (p<0.01), indicating a reduced activation. An enhanced efferocytosis of apoptotic neutrophils by macrophages was observed and accompanied by higher IL-10 and decreased TNF alpha secretion (p<0.01). In vivo, similar results were noted, including reduction of neutrophil migration, protein exudation, and cytokine release (p<0.01). Also, the extract increased fibroblast proliferation and promoted skin wound healing (p<0.01). No signs of toxicity or genotoxicity were observed for the extract. Conclusion. S. diploconos fruit extract is anti-inflammatory by modulating neutrophil migration/activation as well macrophage-dependent efferocytosis and inflammatory mediator release. It also indicates its potential use as a healing agent. Finally, the absence of acute toxic and genotoxic effects reinforces its possible use as medicinal product.

引用

页数：13

共 45 条

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