Identification of the exported proteins of the oral opportunistic pathogen Actinobacillus actinomycetemcomitans by using alkaline phosphatase fusions

被引：8
作者：
Ward, J
Fletcher, J
Nair, SP
Wilson, M
Williams, RJ
Poole, S
Henderson, B
机构：
[1] UCL, Dept Biochem & Mol Biol, Mol Microbiol Grp, London WC1E 6BT, England
[2] UCL, Eastman Dent Inst, Cellular Microbiol Res Grp, London WC1X 8LD, England
[3] Natl Inst Biol Stand & Controls, Div Endocrinol, Potters Bar EN6 3QG, Herts, England
来源：
INFECTION AND IMMUNITY | 2001年 / 69卷 / 04期
关键词：
D O I：
10.1128/IAI.69.4.2748-2752.2001
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
A phoA fusion library of Actinobacillus actinomycetemcomitans genomic DNA has been screened to identify genes encoding exported and secreted proteins. A total of 8,000 colonies were screened, and 80 positive colonies were detected. From these, 48 genes were identified with (i) more than half having homology to known or hypothetical Haemophilus influenzae genes, (ii) 14 having no ascribed function, and (iii) 4 having very limited or no homology to known genes. The proteins encoded by these genes may, by virtue of their presence on the cell surface, be novel virulence determinants.
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收藏
页码：2748 / 2752
页数：5
相关论文
共 18 条
  • [1] Utilization of alkaline phosphatase fusions to identify secreted proteins, including potential efflux proteins and virulence factors from Helicobacter pylori
    Bina, JE
    Nano, F
    Hancock, REW
    [J]. FEMS MICROBIOLOGY LETTERS, 1997, 148 (01) : 63 - 68
  • [2] The minimal gene set member msrA, encoding peptide methionine sulfoxide reductase, is a virulence determinant of the plant pathogen Erwinia chrysanthemi
    El Hassouni, M
    Chambost, JP
    Expert, D
    Van Gijsegem, F
    Barras, F
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (03) : 887 - 892
  • [3] Common themes in microbial pathogenicity revisited
    Finlay, BB
    Falkow, S
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1997, 61 (02) : 136 - +
  • [4] GUITIERREZ C, 1987, J MOL BIOL, V195, P289
  • [5] FUSIONS OF SECRETED PROTEINS TO ALKALINE-PHOSPHATASE - AN APPROACH FOR STUDYING PROTEIN SECRETION
    HOFFMAN, CS
    WRIGHT, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (15) : 5107 - 5111
  • [6] THE POTENT BONE-RESORBING MEDIATOR OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS IS HOMOLOGOUS TO THE MOLECULAR CHAPERONE GROEL
    KIRBY, AC
    MEGHJI, S
    NAIR, SP
    WHITE, P
    REDDI, K
    NISHIHARA, T
    NAKASHIMA, K
    WILLIS, AC
    SIM, R
    WILSON, M
    HENDERSON, B
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1995, 96 (03) : 1185 - 1194
  • [7] MANOIL C, 1991, METHOD CELL BIOL, V34, P61
  • [8] Identification of genes coding for exported proteins of Actinobacillus actinomycetemcomitans
    Mintz, KP
    Fives-Taylor, PM
    [J]. INFECTION AND IMMUNITY, 1999, 67 (11) : 6217 - 6220
  • [9] NEWMAN HN, 1999, DENTL PLAQUE REVISIT
  • [10] GENETIC IDENTIFICATION OF EXPORTED PROTEINS IN STREPTOCOCCUS-PNEUMONIAE
    PEARCE, BJ
    YIN, YB
    MASURE, HR
    [J]. MOLECULAR MICROBIOLOGY, 1993, 9 (05) : 1037 - 1050
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