Synthesis and characterization of a biocompatible copolymer to be used as cell culture support

被引：4

作者：

Becerra, Natalia ^{[1
,2
]}

Restrepo, Litz Marina ^{[2
]}

Lopez, Betty Lucy ^{[1
]}

机构：

[1] Univ Antioquia, Grp Ciencia Mat, Medellin, Colombia

[2] Univ Antioquia, Grp Ingn Tejidos & Terapias Celulares, Medellin, Colombia

来源：

MACROMOLECULAR SYMPOSIA | 2007年 / 258卷

关键词：

biocompatibility; biomaterials; hydrogels; stimuli-sensitive polymers; tissue engineering;

D O I：

10.1002/masy.200751204

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

N-isopropylacrylamide (NIPAAm)/butylacrylate (BAc) copolymer was synthesized by emulsion polymerization in order to use it as a cell culture surface for corneal epithelium biosubstitutes. Results showed that the obtained polymers were thermosensitive hydrogels. The copolymer was characterized by Fourier transform infrared (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and swelling degree. 3T3 Swiss cells were used as feeder layers and they were used to test the cytotoxic effect of the hydrogels. The conditions to isolate, cultivate, expand and cryo-preserve human oral mucosal cells were established and analysis of several morphological and immuno-histochemical characteristics of the cultivated oral mucous were made.

引用

页码：30 / 37

页数：8

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