A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro

被引：35
作者：
Tu, Jian-Qiu [1 ]
Liu, Hui-Ping [1 ]
Wen, Ya-Hui [1 ]
Chen, Pei [1 ]
Liu, Zi-Tian [1 ]
机构：
[1] Tianjin Univ Sci & Technol, Minist Educ China, Coll Food Sci & Engn, State Key Lab Food Nutr & Safety,Key Lab Food Nut, 29,13th Ave,Tianjin Econ & Technol Dev, Tianjin 300457, Peoples R China
来源：
JOURNAL OF FOOD BIOCHEMISTRY | 2021年 / 45卷 / 09期
基金：
中国国家自然科学基金;
关键词：
antioxidant activity; Hericium erinaceus; polysaccharides; structure characterization; thermal stability; LIONS MANE MUSHROOM; FRUITING BODIES; SIGNALING PATHWAYS; OXIDATIVE STRESS; FR; PERS; MYCELIUM; OLIGOSACCHARIDES; EXTRACTION; FRACTION; COLITIS;
D O I：
10.1111/jfbc.13871
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study. We researched its structure and thermal stabilities, and further studied its antioxidant activities in vitro. The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography. Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01. Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing alpha-configuration, mainly consisting of alpha-1-4-Fuc and alpha-1-6-Gal as the main chain, with -> 3,6)-alpha-D-Man-(1 -> and -> 1,6)-Glc was branched, with alpha-D-GlcpA-(1 as T-terminal. The specific rotation of HEP was +55 degrees; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93celcius. In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals. It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% +/- 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc). Therefore, it was revealed that HEP might be used as a natural antioxidant component. Practical applications A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties. This research proved the potential of HEP in anti-oxidation and provided the possibility of developing new natural anti-oxidation products.
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页数：13
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