SHCBP1 is over-expressed in breast cancer and is important in the proliferation and apoptosis of the human malignant breast cancer cell line

被引：72

作者：

Feng, Wen ^{[1
]}

Li, Hong-chang ^{[1
]}

Xu, Ke ^{[2
,3
]}

Chen, Ya-feng ^{[1
]}

Pan, Li-yun ^{[1
]}

Mei, Yi ^{[1
]}

Cai, Han ^{[1
]}

Jiang, Yi-ming ^{[1
]}

Chen, Teng ^{[1
]}

Feng, Dian-xu ^{[1
]}

机构：

[1] Shanghai Univ Tradit Chinese Med, Putuo Hosp, Dept Gen Surg, 164 Lanxi Rd, Shanghai 200062, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, Putuo Hosp, Cent Lab, Shanghai 200062, Peoples R China

[3] Shanghai Univ Tradit Chinese Med, Putuo Hosp, Intervent Canc Inst Chinese Integrat Med, Shanghai 200062, Peoples R China

来源：

GENE | 2016年 / 587卷 / 01期

关键词：

SHCBP1; Breast cancer; CRISPR/CAS9; Proliferation; INSIGHTS;

D O I：

10.1016/j.gene.2016.04.046

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: SHC SH2-binding protein 1, a member of Src homolog and collagen homolog (Shc) family, has been recently identified in different contexts in unbiased screening assays. It has been reported to be over-expressed in several malignant cancers. Methods: Immunohistochemistry of SHCBP1 on 128 breast cancer tissues and adjacent normal tissues were used to evaluate the prognostic significance of SHCBP1. Survival analyses were performed by Kaplan-Meier method. CRISPR/CAS9 method was used to knockout SHCBP1 expression. CRISPR/CAS9 technology was used to knockout SHCBP1 in 2 breast cancer cell lines. MTT assay, BrdU assay, colony formation assay, cell cycle assay and apoptosis analysis in MCF-7 and MDA-MB-231 cell lines were carried out to evaluate the effects of SHCBP1 on breast cancer in vitro. Results: Immunohistochemical analysis revealed SHCBP1 was significantly up-regulated in breast cancer tissues compared with adjacent normal tissues (82 of 128, 64%). Over-expressed SHCBP1 was correlated with advanced clinical stage and poorer survival. Ablation of SHCBP1 inhibited the proliferation in vitro. SHCBP1 knockout increased cyclin-dependent kinase inhibitor p21, and decreased the Cyclin B1 and CDK1. Conclusion: Our study suggests SHCBP1 is dysregulated expressed in breast cancer and plays a critical role in cancer progression, which can be a potential prognosis predictor of breast cancer. (C) 2016 Published by Elsevier B.V.

引用

页码：91 / 97

页数：7

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