Structures of SAS-6 Suggest Its Organization in Centrioles

被引：241

作者：

van Breugel, Mark ^{[1
]}

Hirono, Masafumi ^{[2
]}

Andreeva, Antonina ^{[1
]}

Yanagisawa, Haru-aki ^{[2
]}

Yamaguchi, Shoko ^{[2
]}

Nakazawa, Yuki ^{[2
]}

Morgner, Nina ^{[3
]}

Petrovich, Miriana ^{[1
]}

Ebong, Ima-Obong ^{[3
]}

Robinson, Carol V. ^{[3
]}

Johnson, Christopher M. ^{[1
]}

Veprintsev, Dmitry ^{[1
]}

Zuber, Benoit ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] Univ Tokyo, Dept Biol Sci, Tokyo 1130033, Japan

[3] Univ Oxford, Chem Res Lab, Oxford, England

来源：

SCIENCE | 2011年 / 331卷 / 6021期

关键词：

CRYSTAL-STRUCTURE; BASAL BODY; CENTROSOME DUPLICATION; 9-FOLD SYMMETRY; PROTEIN; PROCENTRIOLE; CARTWHEEL; CHLAMYDOMONAS; BIOGENESIS; MECHANISMS;

D O I：

10.1126/science.1199325

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.

引用

页码：1196 / 1199

页数：4

共 34 条

[1] MORPHOGENESIS OF BASAL BODIES AND ACCESSORY STRUCTURES OF CORTEX OF CILIATED PROTOZOAN TETRAHYMENA PYRIFORMIS [J].