Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase

被引：26

作者：

Berezovskaya, Yana ^{[2
]}

Armstrong, Craig T. ^{[1
]}

Boyle, Aimee L. ^{[1
]}

Porrini, Massimiliano ^{[2
]}

Woolfson, Derek N. ^{[1
,3
]}

Barran, Perdita E. ^{[2
]}

机构：

[1] Univ Bristol, Sch Chem, Bristol, Avon, England

[2] Univ Edinburgh, Sch Chem, Edinburgh, Midlothian, Scotland

[3] Univ Bristol, Dept Biochem, Bristol, Avon, England

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 01期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1039/c0cc02445g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys(2)His(2) zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc >= cobalt >> copper >> calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.

引用

页码：412 / 414

页数：3

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