Quantitative analysis of surface-located triacylglycerol in intact emulsion particles

被引：4

作者：

Boyle-Roden, E ^{[1
]}

Khan, MA ^{[1
]}

机构：

[1] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2001年 / 49卷 / 04期

关键词：

D O I：

10.1021/jf001205u

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The amount of triacylglycerol (TG) in the surface monolayer of intact phospholipid-stabilized emulsions was determined using C-13 nuclear magnetic resonance (C-13 NMR). C-13 NMR spectra of emulsions composed of bulk long-chain or medium-chain TG were prepared with [C-13(3)]-carbonyl-enriched triolein, tripalmitin, or trioctanoin, and were analyzed and compared with NMR spectra of phosphatidylcholine vesicles with and without added TG. Identification of carbonyl peaks intermediate between those of phosphatidylcholine carbonyls and bulk. TG confirmed the presence of surface TG in each emulsion. The surface of emulsions contained 2.2 mol % tripalmitin and 1.4 mol re triolein, but significantly more medium-chain TG, 9.1 mol % trioctanoin, as predicted by measurements of TG in phospholipid vesicles. Thus, medium-chain TGs are more accessible than long-chain TGs to enzymes or pro-oxidants in the continuous phase of phospholipid-stabilized emulsion systems. The quantitative determination of surface-located TG in intact particles will advance the understanding of emulsion colloidal properties, physicochemical stability, and metabolic behavior.

引用

页码：2014 / 2021

页数：8

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