Molecular identification of Giardia duodenalis isolates from humans, dogs, cats and cattle from the state of Sao Paulo, Brazil, by sequence analysis of fragments of glutamate dehydrogenase (gdh) coding gene

被引：101

作者：

Souza, Silvio L. P. ^{[1
]}

Gennari, Solange M. ^{[1
]}

Richtzenhain, Leonardo J. ^{[1
]}

Pena, Hilda F. J. ^{[1
]}

Funada, Mikaela R. ^{[1
]}

Cortez, Adriana ^{[1
]}

Gregori, Fabio ^{[1
]}

Soares, Rodrigo M. ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent Saude Anim, BR-05508900 Sao Paulo, Brazil

来源：

VETERINARY PARASITOLOGY | 2007年 / 149卷 / 3-4期

基金：

巴西圣保罗研究基金会;

关键词：

Giardia duodenalis; domestic animals; humans; glutamate dehydrogenase; molecular identification; Sequencing; Brazil;

D O I：

10.1016/j.vetpar.2007.08.019

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The nucleotide sequence of glutamate dehydrogenase (gdh) coding genes were obtained from cysts of Giardia duodenalis isolated from feces of naturally infected cats (n = 19), dogs (n = 27), humans (n = 37) and cattle (n = 5). The samples were from several municipalities within the state of Sao Paulo, Brazil and were collected from January 2004 to August 2006. Sequences analysis of the 37 specimens recovered from humans revealed 29 G. duodenalis assemblage All and 8 G. duodenalis assemblage B. Among samples from cats, I I were classified into assemblage F and 8 into assemblage Ad. Only the host-adapted assemblages C and D were detected in samples from dogs (7 and 20, respectively). Among the samples from cattle, the genotype livestock was found in four samples and the assemblage AI was detected in one sample. The molecular identification of assemblages of G. duodenalis isolates from different hosts reveals that genetic diversity of this protozoon in Brazil is similar to that of Giardias from other parts of the world. (c) 2007 Elsevier B.V All rights reserved.

引用

页码：258 / 264

页数：7

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