Water-Soluble Polysaccharides from Leaves of Abelmoschus esculentus: Purification, Characterization, and Antioxidant Activity

被引：12
作者：
Li, Q. [1 ]
Zhao, T. [2 ]
Bai, S. Q. [3 ]
Mao, G. H. [4 ]
Zou, Y. [1 ]
Feng, W. W. [1 ]
Wang, W. [1 ]
Huang, J. [2 ]
Wu, X. S. [2 ]
Yang, L. Q. [2 ]
Wu, X. Y. [4 ]
机构：
[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
[2] Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China
[3] Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China
[4] Jiangsu Univ, Sch Environm, Zhenjiang 212013, Peoples R China
来源：
CHEMISTRY OF NATURAL COMPOUNDS | 2017年 / 53卷 / 03期
关键词：
okra leaves; polysaccharides; purification; characterization; antioxidant activity; IN-VITRO; EXTRACTS; L; FLAVONOIDS;
D O I：
10.1007/s10600-017-2011-6
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Two water-soluble polysaccharides were obtained from the leaves of Abelmoschus esculentus using aqueous extraction and purification with DEAE-52 and Sephadex G-100. AEP-11 (1.9 x 10(5) Da) consisted of D-arabinose, D-xylose, D-glucose, D-mannose, and D-galactose in a 2.9:3.1:0.3:0.4:3.4 ratio. AEP-22 (1.6 x 10(6) Da) consisted of D-arabinose, xylose, D-mannose, and galactose in a 4.7:0.8:0.6:3.9 ratio. AFM and Congo red indicated that the enhanced flexibilities of AEP-11 and AEP-22 chains could lead to agglomerated morphologies and may further confirm random coils instead of triple helical conformations. CD revealed the presence of disordered structure in AEP-22 compared with the ordered structure in AEP-11. In antioxidant activity assay, AEP, AEP-11, and AEP-22 all exhibited strong radical-scavenging capacities (AEP > AEP-11 > AEP-22) and obvious reducing power.
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页码：412 / 416
页数：5
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