Site-specific labeling of proteins with small molecules in live cells

被引：241
|
作者：
Chen, I [1 ]
Ting, AY [1 ]
机构：
[1] MIT, Dept Chem, Cambridge, MA 02139 USA
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 2005年 / 16卷 / 01期
关键词：
D O I：
10.1016/j.copbio.2004.12.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The principal bottleneck for the utilization of small-molecule probes in live cells is the shortage of methodologies for targeting them with very high specificity to biological molecules or compartments of interest. Recently developed methods for labeling proteins with small-molecule probes in cells employ special protein or peptide handles that recruit small-molecule ligands, harness enzymes to catalyze small-molecule conjugation or hijack the cell's protein translation machinery.
引用
收藏
页码：35 / 40
页数：6
相关论文
共 50 条
  • [21] Site-Specific Labeling of Endogenous Proteins Using CoLDR Chemistry
    Reddi, Rambabu N.
    Rogel, Adi
    Resnick, Efrat
    Gabizon, Ronen
    Prasad, Pragati Kishore
    Gurwicz, Neta
    Barr, Haim
    Shulman, Ziv
    London, Nir
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2021, 143 (48) : 20095 - 20108
  • [22] Concerted, Rapid, Quantitative, and Site-Specific Dual Labeling of Proteins
    Sachdeva, Amit
    Wang, Kaihang
    Elliott, Thomas
    Chin, Jason W.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (22) : 7785 - 7788
  • [23] Site-specific Bimodal Labeling of Proteins on Cysteine Residues with Chlorotetrazines
    Canovas, C.
    Moreau, M.
    Bernhard, C.
    Denat, F.
    Goncalves, V.
    EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2017, 44 : S559 - S559
  • [24] SITE-SPECIFIC ISOTOPIC LABELING OF PROTEINS FOR NMR-STUDIES
    ELLMAN, JA
    VOLKMAN, BF
    MENDEL, D
    SCHULTZ, PG
    WEMMER, DE
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (20) : 7959 - 7961
  • [25] GFP for EM: Site-Specific Labeling of Proteins for Electron Microscopy
    Dambacher, Corey M.
    Lander, Gabriel C.
    BIOPHYSICAL JOURNAL, 2015, 108 (02) : 189A - 190A
  • [26] Site-Specific Dual Labeling of Proteins on Cysteine Residues with Chlorotetrazines
    Canovas, Coline
    Moreau, Mathieu
    Bernhard, Claire
    Oudot, Alexandra
    Guillemin, Melanie
    Denat, Franck
    Goncalves, Victor
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 57 (33) : 10646 - 10650
  • [27] Site-Specific Fluorescent Labeling of Nascent Proteins on the Translating Ribosome
    Saraogi, Ishu
    Zhang, Dawei
    Chandrasekaran, Sandhya
    Shan, Shu-ou
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (38) : 14936 - 14939
  • [28] Bioorthogonal Chemistry for Site-Specific Labeling and Surface Immobilization of Proteins
    Chen, Yong-Xiang
    Triola, Gemma
    Waldmann, Herbert
    ACCOUNTS OF CHEMICAL RESEARCH, 2011, 44 (09) : 762 - 773
  • [29] Monitoring Protein Misfolding by Site-Specific Labeling of Proteins In Vivo
    Hsieh, Tzung-yang
    Nillegoda, Nadinath B.
    Tyedmers, Jens
    Bukau, Bernd
    Mogk, Axel
    Kramer, Guenter
    PLOS ONE, 2014, 9 (06):
  • [30] An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides
    Tominaga, Jo
    Kemori, Yoshinori
    Tanaka, Yusuke
    Maruyama, Tatsuo
    Kamiya, Noriho
    Goto, Masahiro
    CHEMICAL COMMUNICATIONS, 2007, (04) : 401 - 403
12345