Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles

被引：83

作者：

Kim, Dokyoung ^{[1
,2
]}

Kang, Jinyoung ^{[3
]}

Wang, Taejun ^{[4
]}

Ryu, Hye Gun ^{[5
]}

Zuidema, Jonathan M. ^{[6
]}

Joo, Jinmyoung ^{[7
,8
]}

Kim, Muwoong ^{[1
]}

Huh, Youngbuhm ^{[1
]}

Jung, Junyang ^{[1
]}

Ahn, Kyo Han ^{[5
]}

Kim, Ki Hean ^{[9
]}

Sailor, Michael J. ^{[10
]}

机构：

[1] Kyung Hee Univ, Dept Anat & Neurobiol, Coll Med, 26 Kyungheedae Ro, Seoul 02447, South Korea

[2] Kyung Hee Univ, Ctr Converging Humanities, 26 Kyungheedae Ro, Seoul 02447, South Korea

[3] Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 USA

[4] Pohang Univ Sci & Technol POSTECH, Div Integrat Biosci & Biotechnol, 77 Cheongam Ro, Pohang 37673, Gyungbuk, South Korea

[5] Pohang Univ Sci & Technol POSTECH, Dept Chem, 77 Cheongam Ro, Pohang 37673, Gyungbuk, South Korea

[6] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[7] Univ Ulsan, Dept Convergence Med, Coll Med, 88 Olymp Ro 43-Gil, Seoul 05505, South Korea

[8] Asan Med Ctr, Asan Inst Life Sci, Biomed Engn Res Ctr, 88 Olymp Ro 43-Gil, Seoul 05505, South Korea

[9] Pohang Univ Sci & Technol POSTECH, Div Integrat Biosci & Biotechnol, Dept Mech Engn, 77 Cheongam Ro, Pohang 37673, Gyungbuk, South Korea

[10] Univ Calif San Diego, Dept Chem & Biochem, Dept Nanoengn, La Jolla, CA 92093 USA

来源：

ADVANCED MATERIALS | 2017年 / 29卷 / 39期

基金：

美国国家科学基金会;

关键词：

cancer diagnostics; iRGD targeting peptide; nanomedicine; photoluminescence; SEMICONDUCTOR QUANTUM DOTS; 2ND-HARMONIC GENERATION; FLUORESCENCE; MICROSCOPY; DELIVERY; NANOCRYSTALS; ABSORPTION; EMISSION; EXCITATION; PEPTIDE;

D O I：

10.1002/adma.201703309

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A major obstacle in luminescence imaging is the limited penetration of visible light into tissues and interference associated with light scattering and autofluorescence. Near-infrared (NIR) emitters that can also be excited with NIR radiation via two-photon processes can mitigate these factors somewhat because they operate at wavelengths of 650-1000 nm where tissues are more transparent, light scattering is less efficient, and endogenous fluorophores are less likely to absorb. This study presents photolytically stable, NIR photoluminescent, porous silicon nanoparticles with a relatively high two-photon-absorption cross-section and a large emission quantum yield. Their ability to be targeted to tumor tissues in vivo using the iRGD targeting peptide is demonstrated, and the distribution of the nanoparticles with high spatial resolution is visualized.

引用

页数：8

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