Anaerobic conditions to reduce oxidation of proteins and to accelerate the copper-catalyzed "Click" reaction with a water-soluble bis(triazole) ligand

被引：35

作者：

Kumar, Amit ^{[1
]}

Li, King ^{[2
]}

Cai, Chengzhi ^{[1
]}

机构：

[1] Univ Houston, Dept Chem, Houston, TX 77204 USA

[2] Methodist Hosp, Res Inst, Dept Radiol, Houston, TX 77030 USA

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 11期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

AZIDE-ALKYNE CYCLOADDITION; TERMINAL ALKYNES; IN-VIVO; AMINOGUANIDINE;

D O I：

10.1039/c0cc05376g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oxidation of protein (bovine albumin serum) by air still occurred under the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction conditions even in the presence of a Cu(I)-stabilizing tris(triazole) ligand. Anaerobic conditions not only avoided the oxidation of the protein, but also greatly accelerated the CuAAC reaction using a water-soluble bis(triazole) Cu(I) ligand.

引用

收藏

页码：3186 / 3188

页数：3

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