Genetic diversity analysis of radish germplasm with RAPD, AFLP and SRAP markers

Randomly amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) markers were employed for germplasm identification and genetic diversity analysis of 17 radish accessions, which consisted of eleven Chinese varieties, five foreign cultivated varieties and one wild relative. The RAPD marker was the most polymorphic (93.4%) among the three marker types. An average of 23.25 polymorphic fragments amplified with each AFLP primer combination was obtained with a relatively low polymorphic ratio. The dendrogram based on RAPD and SRAP data showed high accordance with the classification according to the morphological traits. The genetic diversity of radish germplasm was highly associated with the color of fresh root skin and their origin. Moreover, as for revealing the genetic relationship of different radish accessions, the data from multiple marker systems was more reliable than that from only one type of marker analysis.