Molecular characterization of Bacillus thuringiensis strains to control Spodoptera eridania (Cramer) (Lepidoptera: Noctuidae) population

被引:0
作者
Bittencourt Machado, Deborah Heloisa [1 ]
do Livramento, Kalynka Gabriela [2 ]
Flausino Maximo, Wesley Pires [2 ]
Negri, Barbara Franca [3 ]
Paiva, Luciano Vilela [4 ]
Valicente, Fernando Hercos [1 ]
机构
[1] Embrapa Milho & Sorgo, NBA, Sete Lagoas, MG, Brazil
[2] Univ Fed Lavras UFLA, Lab Cent Biol Mol, Lavras, MG, Brazil
[3] Univ Fed Sao Joao del Rei UFSJ, Sao Joao Del Rei, MG, Brazil
[4] Univ Fed Lavras UFLA, Dept Quim, Lavras, MG, Brazil
关键词
Entomopathogenic bacteria; Insect pest; Molecular typing; Toxicity; REP-PCR; ERIC-PCR; DIVERSITY; FRUGIPERDA; CRY;
D O I
10.1590/1806-9665-RBENT-2019-47
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The main objective of this study was to characterize the toxicity and genetic divergence of 18 Bacillus thuringiensis strains in the biological control of Spodoptera eridania. Bacterial suspensions were added to the S. eridania diet. Half of the selected B. thuringiensis strains caused high mortality seven days after infection. The genetic divergence of B. thuringiensis strains was assessed based on Enterobacterial Repetitive Intergenic Consensus (ERIC) and Repetitive Extragenic Palindromic (REP) sequences, and five phylogenetic groups were formed. Despite their genetic diversity B. thuringiensis strains did not show any correlation between the collection sites and toxicity to larvae. Some B. thuringiensis strains are highly toxic to S. eridania, thus highlighting the potential of their endotoxins as biopesticides.
引用
收藏
页数:6
相关论文
共 39 条
  • [1] Phylogenetic relationships of fluorescent Pseudomonad isolates associated with bacterial canker of stone fruit trees in Kurdistan province, Iran
    Ahmadi, Sakineh
    Harighi, Behrouz
    Abdollahzadeh, Jafar
    [J]. EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2018, 150 (03) : 679 - 689
  • [2] Low susceptibility of Spodoptera cosmioides, Spodoptera eridania and Spodoptera frugiperda (Lepidoptera: Noctuidae) to genetically-modified soybean expressing Cry1Ac protein
    Bernardi, Oderlei
    Sorgatto, Rodrigo J.
    Barbosa, Alexandre D.
    Domingues, Felipe A.
    Dourado, Patrick M.
    Carvalho, Renato A.
    Martinelli, Samuel
    Head, Graham P.
    Omoto, Celso
    [J]. CROP PROTECTION, 2014, 58 : 33 - 40
  • [4] Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control
    Bravo, Alejandra
    Gill, Sarjeet S.
    Soberon, Mario
    [J]. TOXICON, 2007, 49 (04) : 423 - 435
  • [5] Bacillus thuringiensis: A story of a successful bioinsecticide
    Bravo, Alejandra
    Likitvivatanavong, Supaporn
    Gill, Sarjeet S.
    Soberon, Mario
    [J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2011, 41 (07) : 423 - 431
  • [6] CAB International-CABI, 2018, INV SPEC COMP SPOD E
  • [7] Genomic diversity and relationship of Bacillus thuringiensis and Bacillus cereus by multi-REP-PCR fingerprinting
    Cherif, Ameur
    Ettoumi, Besma
    Raddadi, Noura
    Daffonchio, Daniele
    Boudabous, Abdellatif
    [J]. CANADIAN JOURNAL OF MICROBIOLOGY, 2007, 53 (03) : 343 - 350
  • [8] CONAB (Companhia Nacional de Abastecimento), 2018, AC SAFR BRAS GRAOS S
  • [9] Selection and molecular characterization of Bacillus thuringiensis isolates for controlling Spodoptera spp.
    Constanski, Kelly Christiane
    Zorzetti, Janaina
    Vilas Boas, Gislayne Trindade
    Scaramal Ricieto, Ana Paula
    Pires Fazion, Fernanda Aparecida
    Boas, Laurival Vilas
    Monnerat, Rose Gomes
    Oliveira Janeiro Neves, Pedro Manuel
    [J]. PESQUISA AGROPECUARIA BRASILEIRA, 2015, 50 (08) : 730 - 734
  • [10] Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins
    Crickmore, N
    Zeigler, DR
    Feitelson, J
    Schnepf, E
    Van Rie, J
    Lereclus, D
    Baum, J
    Dean, DH
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (03) : 807 - +