Single Cell Physiology

被引：2

作者：

Neveu, Pierre ^{[1
]}

Sinha, Deepak Kumar ^{[2
]}

Kettunen, Petronella ^{[3
]}

Vriz, Sophie ^{[4
]}

Jullien, Ludovic ^{[5
]}

Bensimon, David ^{[6
,7
]}

机构：

[1] Univ Calif Santa Barbara, Kavli Inst Theoret Phys, Santa Barbara, CA 93106 USA

[2] Ecole Normale Super, UMR, Lab Phys Stat, Paris, France

[3] Univ Calif Los Angeles, Dept Physiol Sci, Los Angeles, CA 90024 USA

[4] Inserm U770, Hemostase Dynam Cellulaire Vasculaire, Le Kremlin Bicetre, France

[5] Ecole Normale Super, UMR 8640, Dept Chim, Paris, France

[6] Ecole Normale Super, UMR 8550, Lab Phys Stat, Paris, France

[7] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90024 USA

来源：

SINGLE MOLECULE SPECTROSCOPY IN CHEMISTRY, PHYSICS AND BIOLOGY | 2010年 / 96卷

基金：

美国国家科学基金会;

关键词：

ZEBRAFISH EMBRYOS; GENE-EXPRESSION; RETINOIC ACID; CAGED-ATP; MAMMALIAN-CELLS; DENDRITIC SPINES; LIGHT; PHOTOLYSIS; RECEPTOR; MOLECULES;

D O I：

10.1007/978-3-642-02597-6_16

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The possibility to control at specific times and specific places the activity of biomolecules (enzymes, transcription factors, RNA, hormones, etc.) is opening up new opportunities in the study of physiological processes at the single cell level in a live organism. Most existing gene expression systems allow for tissue specific induction upon feeding the organism with exogenous inducers (e.g., tetracycline). Local genetic control has earlier been achieved by micro-injection of the relevant inducer/repressor molecule, but this is an invasive and possibly traumatic technique. In this chapter, we present the requirements for a noninvasive optical control of the activity of biomolecules and review the recent advances in this new field of research.

引用

页码：305 / +

页数：3

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