SFPQ Promotes Lung Cancer Malignancy via Regulation of CD44 v6 Expression

被引：8

作者：

Yang, Libang ^{[1
]}

Yang, Jianbo ^{[2
,3
]}

Jacobson, Blake ^{[4
]}

Gilbertsen, Adam ^{[1
]}

Smith, Karen ^{[1
]}

Higgins, LeeAnn ^{[5
]}

Guerrero, Candace ^{[5
]}

Xia, Hong ^{[1
]}

Henke, Craig A. ^{[1
]}

Lin, Jizhen ^{[3
,6
]}

机构：

[1] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA

[2] Univ Minneapolis, Sch Med, Dept Lab Med & Pathol, Minneapolis, MN USA

[3] Fujian Med Univ Union Hosp, Canc Ctr, Fuzhou, Peoples R China

[4] Univ Minnesota, Sch Med, Hematol Oncol & Transplantat, Minneapolis, MN USA

[5] Univ Minnesota, Ctr Mass Spectrometry & Prote, St Paul, MN USA

[6] Univ Minnesota, Canc Ctr, Dept Otolaryngol, Immunotherapy Res Lab, Minneapolis, MN 55455 USA

来源：

FRONTIERS IN ONCOLOGY | 2022年 / 12卷

基金：

美国国家卫生研究院;

关键词：

mesenchymal stem cells (MSCs); lung non-small cell (NSC) cancer; nuclear fraction; quantitative proteomics; ingenuity pathway analysis; SFPQ; CD44v6; COLORECTAL-CANCER; TUMOR PROGRESSION; CELLS; PROTEOMICS; PROTEINS; IDENTIFICATION; METASTASIS; INVASION; BINDING; MIGRATION;

D O I：

10.3389/fonc.2022.862250

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Mesenchymal stem cells (MSCs) contribute to tumor pathogenesis and elicit antitumor immune responses in tumor microenvironments. Nuclear proteins might be the main players in these processes. In the current study, combining spatial proteomics with ingenuity pathway analysis (IPA) in lung non-small cell (NSC) cancer MSCs, we identify a key nuclear protein regulator, SFPQ (Splicing Factor Proline and Glutamine Rich), which is overexpressed in lung cancer MSCs and functions to promote MSCs proliferation, chemical resistance, and invasion. Mechanistically, the knockdown of SFPQ reduces CD44v6 expression to inhibit lung cancer MSCs stemness, proliferation in vitro, and metastasis in vivo. The data indicates that SFPQ may be a potential therapeutic target for limiting growth, chemotherapy resistance, and metastasis of lung cancer.

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页数：13

相关论文

共 74 条

[1] Role of CD44s and CD44v6 on human breast cancer cell adhesion, migration, and invasion [J].

Afify, Alaa ;

Purnell, Phillip ;

Nguyen, Laura .

EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2009, 86 (02) :95-100

[2] Cell Type-Specific TGF-β Mediated EMT in 3D and 2D Models and Its Reversal by TGF-β Receptor Kinase Inhibitor in Ovarian Cancer Cell Lines [J].

Al Ameri, Wafa ;

Ahmed, Ikhlak ;

Al-Dasim, Fatima M. ;

Mohamoud, Yasmin Ali ;

Al-Azwani, Iman K. ;

Malek, Joel A. ;

Karedath, Thasni .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (14)

[3] Integrated nuclear proteomics and transcriptomics identifies S100A4 as a therapeutic target in acute myeloid leukemia [J].

Alanazi, Bader ;

Munje, Chinmay R. ;

Rastogi, Namrata ;

Williamson, Andrew J. K. ;

Taylor, Samuel ;

Hole, Paul S. ;

Hodges, Marie ;

Doyle, Michelle ;

Baker, Sarah ;

Gilkes, Amanda F. ;

Knapper, Steven ;

Pierce, Andrew ;

Whetton, Anthony D. ;

Darley, Richard L. ;

Tonks, Alex .

LEUKEMIA, 2020, 34 (02) :427-440

[4] Subnuclear Proteomics in Colorectal Cancer IDENTIFICATION OF PROTEINS ENRICHED IN THE NUCLEAR MATRIX FRACTION AND REGULATION IN ADENOMA TO CARCINOMA PROGRESSION [J].

Albrethsen, Jakob ;

Knol, Jaco C. ;

Piersma, Sander R. ;

Pham, Thang V. ;

de Wit, Meike ;

Mongera, Sandra ;

Carvalho, Beatriz ;

Verheul, Henk M. W. ;

Fijneman, Remond J. A. ;

Meijer, Gerrit A. ;

Jimenez, Connie R. .

MOLECULAR & CELLULAR PROTEOMICS, 2010, 9 (05) :988-1005

[5] CD44v6 High Membranous Expression Is a Predictive Marker of Therapy Response in Gastric Cancer Patients [J].

Almeida, Gabriela M. ;

Pereira, Carla ;

Park, Ji-Hyeon ;

Lemos, Carolina ;

Campelos, Sofia ;

Gullo, Irene ;

Martins, Diana ;

Goncalves, Gilza ;

Leitao, Dina ;

Luis Neto, Joao ;

Andre, Ana ;

Borges, Clara ;

Almeida, Daniela ;

Lee, Hyuk-Joon ;

Kong, Seong-Ho ;

Kim, Woo Ho ;

Carneiro, Fatima ;

Almeida, Raquel ;

Yang, Han-Kwang ;

Oliveira, Carla .

BIOMEDICINES, 2021, 9 (09)

[6] Quantitative Proteomics Identifies DNA Repair as a Novel Biological Function for Hepatocyte Nuclear Factor 4α in Colorectal Cancer Cells [J].

Babeu, Jean-Philippe ;

Wilson, Samuel D. ;

Lambert, Elie ;

Levesque, Dominique ;

Boisvert, Francois-Michel ;

Boudreau, Francois .

CANCERS, 2019, 11 (05)

[7] Quantitative Nuclear Proteomics Identifies mTOR Regulation of DNA Damage Response [J].

Bandhakavi, Sricharan ;

Kim, Young-Mi ;

Ro, Seung-Hyun ;

Xie, Hongwei ;

Onsongo, Getiria ;

Jun, Chang-Bong ;

Kim, Do-Hyung ;

Griffin, Timothy J. .

MOLECULAR & CELLULAR PROTEOMICS, 2010, 9 (02) :403-414

[8] Concise Review: Aggressive Colorectal Cancer: Role of Epithelial Cell Adhesion Molecule in Cancer Stem Cells and Epithelial-to-Mesenchymal Transition [J].

Boesch, Maximilian ;

Spizzo, Gilbert ;

Seeber, Andreas .

STEM CELLS TRANSLATIONAL MEDICINE, 2018, 7 (06) :495-501

[9] Combined Proteomics and Transcriptomics Identifies Carboxypeptidase B1 and Nuclear Factor κB (NF-κB) Associated Proteins as Putative Biomarkers of Metastasis in Low Grade Breast Cancer [J].

Bouchal, Pavel ;

Dvorakova, Monika ;

Roumeliotis, Theodoros ;

Bortlicek, Zbynek ;

Ihnatova, Ivana ;

Prochazkova, Iva ;

Ho, Jenny T. C. ;

Maryas, Josef ;

Imrichova, Hana ;

Budinska, Eva ;

Vyzula, Rostislav ;

Garbis, Spiros D. ;

Vojtesek, Borivoj ;

Nenutil, Rudolf .

MOLECULAR & CELLULAR PROTEOMICS, 2015, 14 (07) :1814-1830

[10]

Chi C, 2015, EUR REV MED PHARMACO, V19, P241

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