Comparative genomic analyses of β-lactamase (blaCMY-42)-encoding plasmids isolated from wastewater treatment plants in Canada

被引:4
作者
Ajayi, Adeyinka O. [1 ]
Perry, Benjamin J. [1 ]
Yost, Christopher K. [1 ]
Jamieson, Rob C. [2 ]
Hansen, Lisbeth Truelstrup [3 ,4 ]
Rahube, Teddie O. [1 ,5 ]
机构
[1] Univ Regina, Inst Microbial Syst & Soc, Biol Dept, Regina, SK, Canada
[2] Dalhousie Univ, Dept Civil & Resource Engn, Halifax, NS, Canada
[3] Dalhousie Univ, Dept Proc Engn & Appl Sci, Halifax, NS, Canada
[4] Tech Univ Denmark, Natl Food Inst, Lyngby, Denmark
[5] Botswana Int Univ Sci & Technol, Dept Biol Sci & Biotechnol, Palapye, Botswana
基金
加拿大自然科学与工程研究理事会;
关键词
wastewater; bla(CMY-42); resistance plasmids; beta-lactamase; antimicrobial resistance; ESCHERICHIA-COLI; ANTIBIOTIC-RESISTANCE; GENES; IDENTIFICATION; DIVERSITY; SEQUENCES; MOBILITY; ANIMALS; STRAINS;
D O I
10.1139/cjm-2021-0012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Wastewater treatment plants (WWTPs) are useful environments for investigating the occurrence, diversity, and evolution of plasmids encoding clinically relevant antibiotic resistance genes (ARGs). Our objective was to isolate and sequence plasmids encoding meropenem resistance from bacterial hosts within Canadian WWTPs. We used two enrichment culture approaches for primary plasmid isolation, followed by screening for antibiotic resistance, conjugative mobility, and stability in enteric bacteria. Isolated plasmids were sequenced using Illumina MiSeq and Sanger sequencing methods. Bioinformatics analyses resolved a multi-resistance IncF/MOBF12 plasmid, pFEMG (209 357 bp), harbouring resistance genes to beta-lactam (bla(CMY-42), bla(TEM-1 beta), and bla(NDM-5)), macrolide (mphA-mrx-mphR), tetracycline (tetR-tetB-tetC-tetD), trimethoprim (dfrA12), aminoglycoside (aadA2), and sulfonamide (sul1) antibiotic classes. We also isolated an IncI1/MOBP12 plasmid pPIMR (172 280 bp) carrying similar beta-lactamase and a small multi-drug efflux resistance gene cluster (bla(CMY-42)-blc-sugE) to pFEMG. The co-occurrence of different ARGs within a single 24 552 bp cluster in pFEMG - interspersed with transposons, insertion sequence elements, and a class 1 integron - may be of significant interest to human and veterinary medicine. Additionally, the presence of conjugative and plasmid maintenance genes in the studied plasmids corresponded to observed high conjugative transfer frequencies and stable maintenance. Extensive investigation is required to further understand the fitness trade-offs of plasmids with different types of conjugative transfer and maintenance modules.
引用
收藏
页码:737 / 748
页数:12
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