Short-hairpin RNAs delivered by recombinant adeno-associated virus inhibited the replication of influenza A viruses in vitro

被引：1
作者：
Zhang, Gui [1 ]
Ng, Fai [2 ]
Chen, Min [2 ]
机构：
[1] Capital Med Univ, Beijing Tongren Hosp, Dept Lab Med, Beijing 100730, Peoples R China
[2] Univ Hong Kong, Dept Microbiol, Pokfulam, Hong Kong, Peoples R China
来源：
VIROLOGY | 2021年 / 564卷
关键词：
Influenza A virus; Short-hairpin RNAs; AAV; RNAi; Replication; MATRIX;
D O I：
10.1016/j.virol.2021.10.002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Antiviral short-hairpin RNAs (shRNAs) delivered by recombinant adeno-associated virus (rAAV) were investigated for their potential prophylactic and therapeutic applications related to the influenza A virus (IAV). To express shRNAs efficiently, an H1 promoter was inserted into the commercial rAAV2 system. The modified rAAV2 system could express shRNAs, and the purified rAAV was obtained at levels over 10(13) viral genomes/ml and 10(10) viral infection units/ml. The shNP-1496-n and shM2-925 delivered by rAAV could inhibit the replication of the H1N1 and H5N1 virus by targeting the conserved regions of the IAV nucleoprotein and matrix 2 genes in MDCK cells. The shNP-1496-n and shM2-925 expressed by rAAV could provide potent and long-term anti-H5N1 virus effects in rAAV-shRNA-enriched MDCK cells. Our findings provide a rational basis for developing RNA interference for the prevention and therapy of IAV infection.
引用
收藏
页码：46 / 52
页数：7
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共 26 条
  • [1] Current challenges: from the path of "original antigenic sin" towards the development of universal flu vaccines
    Biswas, Asim
    Chakrabarti, Alok K.
    Dutta, Shanta
    [J]. INTERNATIONAL REVIEWS OF IMMUNOLOGY, 2020, 39 (01) : 21 - 36
  • [2] Human immunodeficiency virus type 1 escape from RNA interference
    Boden, D
    Pusch, O
    Lee, F
    Tucker, L
    Ramratnam, B
    [J]. JOURNAL OF VIROLOGY, 2003, 77 (21) : 11531 - 11535
  • [3] Inhibition of influenza A virus by mixed siRNAs, targeting the PA, NP, and NS genes, delivered by hybrid microcarriers
    Brodskaia, Aleksandra, V
    Timin, Alexander S.
    Gorshkov, Andrey N.
    Muslimov, Albert R.
    Bondarenko, Andrei B.
    Tarakanchikova, Yana, V
    Zabrodskaya, Yana A.
    Baranovskaya, Irina L.
    Il'inskaja, Eugenia, V
    Sakhenberg, Elena, I
    Sulthorukov, Gleb B.
    Vasin, Andrey V.
    [J]. ANTIVIRAL RESEARCH, 2018, 158 : 147 - 160
  • [4] Recombinant Adeno-Associated Viral Vectors (rAAV)-Vector Elements in Ocular Gene Therapy Clinical Trials and Transgene Expression and Bioactivity Assays
    Buck, Thilo M.
    Wijnholds, Jan
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (12) : 1 - 52
  • [5] Recombinant adeno-associated virus expressing the receptor-binding domain of severe acute respiratory syndrome coronavirus S protein elicits neutralizing antibodies: Implication for developing SARS vaccines
    Du, Lanying
    He, Yuxian
    Wang, Yija
    Zhang, Haojie
    Ma, Selene
    Wong, Charlotte K. L.
    Wu, Sharon H. W.
    Ng, Fai
    Huang, Jian-Dong
    Yuen, Kwok-Yung
    Jiang, Shibo
    Zhou, Yusen
    Zheng, Bo-Jian
    [J]. VIROLOGY, 2006, 353 (01) : 6 - 16
  • [6] Antiviral effects against influenza A virus infection by a short hairpin RNA targeting the non-coding terminal region of the viral nucleoprotein gene
    Fujimoto, Yoshikazu
    Kyogoku, Kenji
    Takeda, Keiko
    Ozaki, Kinuyo
    Yamamoto, Sayo
    Suyama, Haruka
    Ono, Etsuro
    [J]. JOURNAL OF VETERINARY MEDICAL SCIENCE, 2019, 81 (03) : 383 - 388
  • [7] Preparing intensive care for the next pandemic influenza
    Kain, Taylor
    Fowler, Robert
    [J]. CRITICAL CARE, 2019, 23 (01):
  • [8] Overcoming the phage replication threshold: a mathematical model with implications for phage therapy
    Kasman, LM
    Kasman, A
    Westwater, C
    Dolan, J
    Schmidt, MG
    Norris, JS
    [J]. JOURNAL OF VIROLOGY, 2002, 76 (11) : 5557 - 5564
  • [9] Influenza vaccine: Where are we and where do we go?
    Keshavarz, Mohsen
    Mirzaei, Hamed
    Salemi, Maryam
    Momeni, Fatemeh
    Mousavi, Mohammad Javad
    Sadeghalvad, Mona
    Arjeini, Yaser
    Solaymani-Mohammadi, Farid
    Nahand, Javid Sadri
    Namdari, Haideh
    Mokhtari-Azad, Talat
    Rezaei, Farhad
    [J]. REVIEWS IN MEDICAL VIROLOGY, 2019, 29 (01)
  • [10] Emerging influenza viruses and the prospect of a universal influenza virus vaccine
    Krammer, Florian
    [J]. BIOTECHNOLOGY JOURNAL, 2015, 10 (05) : 690 - U70
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