Strain selection and medium optimization for glucoamylase production from industrial potato waste by Aspergillus niger

被引:26
作者
Izmirlioglu, Gulten [1 ]
Demirci, Ali [1 ,2 ]
机构
[1] Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA
[2] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA
关键词
glucoamylase; Aspergillus niger; industrial waste potato mash; Plackett-Burman; central composite design; SOLID-STATE FERMENTATION; ALPHA-AMYLASE PRODUCTION; STATISTICAL DESIGN; PROCESS PARAMETERS; CULTURE-MEDIUM; LACTIC-ACID; STARCH; SACCHARIFICATION; ETHANOL; ORYZAE;
D O I
10.1002/jsfa.7445
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
BACKGROUND: Glucoamylase is one of the most common enzymes used in the food industry to break down starch into its monomers. Glucoamylase production and its activity are highly dependent on medium composition. Starch is well known as a glucoamylase inducer, and utilization of industrial starchy potato waste is an inexpensive way of improving glucoamylase production. Since glucoamylase production is highly dependent on medium composition, in this study medium optimization for glucoamylase production was considered to enhance glucoamylase activity. RESULTS: Among the evaluated microbial species, Aspergillus niger van Tieghem was found to be the best glucoamylase-producing fungus. The Plackett-Burman design was used to screen various medium ingredients, and malt extract, FeSO4.7H(2)O and CaCl2 center dot 2H(2)O were found to have significant effects on glucoamylase production. Finally, malt extract, FeSO4.7H(2)O and CaCl2.2H(2)O were optimized by using a central composite design of response surface methodology. The results showed that the optimal medium composition for A. niger van Tieghem was 50 g L-1 industrial waste potato mash supplemented with 51.82 g L-1 malt extract, 9.27 g L-1 CaCl2 center dot 2H(2)O and 0.50 g L-1 FeSO4.7H(2)O. CONCLUSION: At the end of optimization, glucoamylase activity and glucose production were improved 126% and 98% compared to only industrial waste potato mash basal medium; 274.4 U mL(-1) glucoamylase activity and 41.7 g L-1 glucose levels were achieved, respectively. (c) 2015 Society of Chemical Industry
引用
收藏
页码:2788 / 2795
页数:8
相关论文
共 36 条
[1]   DIRECT FERMENTATION OF POTATO STARCH TO ETHANOL BY COCULTURES OF ASPERGILLUS-NIGER AND SACCHAROMYCES-CEREVISIAE [J].
ABOUZIED, MM ;
REDDY, CA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1986, 52 (05) :1055-1059
[2]   Purification and properties of two forms of glucoamylase from Aspergillus niger [J].
Amirul, AA ;
Khoo, SL ;
Nazalan, MN ;
Razip, MS ;
Azizan, MN .
FOLIA MICROBIOLOGICA, 1996, 41 (02) :165-174
[3]  
[Anonymous], 1946, Biometrika, DOI [DOI 10.2307/2332195, 10.2307/2332195, DOI 10.1093/BIOMET/33.4.305]
[4]   Glucoamylase production by solid-state fermentation using rice flake manufacturing waste products as substrate [J].
Anto, H ;
Trivedi, UB ;
Patel, KC .
BIORESOURCE TECHNOLOGY, 2006, 97 (10) :1161-1166
[5]  
Arnthong J, 2010, AFR J BIOTECHNOL, V9, P7312
[6]   Pullulan production by Aureobasidium pullulans growing on hydrolysed potato starch waste [J].
Barnett, C ;
Smith, A ;
Scanlon, B ;
Israilides, CJ .
CARBOHYDRATE POLYMERS, 1999, 38 (03) :203-209
[7]  
Bhatti HN, 2007, FOOD TECHNOL BIOTECH, V45, P51
[8]   INFLUENCE OF CARBON AND NITROGEN-SOURCES ON GLUCOAMYLASE PRODUCTION BY ASPERGILLUS IN BATCH PROCESS [J].
CHIQUETTO, ML ;
FACCIOTTI, MCR ;
KILIKIAN, BV ;
SCHMIDELL, W .
BIOTECHNOLOGY LETTERS, 1992, 14 (06) :465-470
[9]   Application of a statistical design to the optimization of culture medium for α-amylase production by Aspergillus niger ATCC 16404 grown on orange waste powder [J].
Djekrif-Dakhmouche, S ;
Gheribi-Aoulmi, B ;
Meraihi, Z ;
Bennamoun, L .
JOURNAL OF FOOD ENGINEERING, 2006, 73 (02) :190-197
[10]  
El-Gendy M. M. A., 2012, Australian Journal of Basic and Applied Sciences, V6, P41