Influence of Pulse Rise Time on the Inactivation of Staphylococcus Aureus by Pulsed Electric Fields

被引：16

作者：

Chen, Jie ^{[1
]}

Zhang, Ruobing ^{[2
]}

Xiao, Jianfu ^{[1
]}

Li, Jing ^{[3
]}

Wang, Liming ^{[2
]}

Guan, Zhicheng ^{[2
]}

MacAlpine, Mark ^{[2
,4
]}

机构：

[1] Tsinghua Univ, Beijing 100084, Peoples R China

[2] Tsinghua Univ, Grad Sch Shenzhen, Lab Adv Technol Elect Engn & Energy, Shenzhen 518055, Peoples R China

[3] China Agr Univ, Beijing 100083, Peoples R China

[4] Harbin Tech Univ, Harbin 150001, Peoples R China

来源：

IEEE TRANSACTIONS ON PLASMA SCIENCE | 2010年 / 38卷 / 08期

关键词：

Fast Fourier transform (FFT); pulsed electric field (PEF); rise time; Staphylococcus aureus; CELLS; ELECTROPORATION; MICROORGANISMS;

D O I：

10.1109/TPS.2010.2050785

中图分类号：

O35 [流体力学]; O53 [等离子体物理学];

学科分类号：

070204 ; 080103 ; 080704 ;

摘要：

In this paper, the effect of rise time (200 ns and 2 mu s, respectively) of rectangular pulse on the inactivation of Staphylococcus aureus has been studied. Apple juice with three different conductivities (1.5 mS/cm, 2.0 mS/cm, and 2.5 mS/cm) was inactivated 75 mu s at an electric field strength of 25 kV/cm, 30 kV/cm, and 35 kV/cm, respectively. The experiment results showed that the pulse with shorter rise time had higher effect of inactivation and lower solution temperature increases after treatment, and the difference between te inactivation effects was more than 0.5 logs in general. Fast Fourier transform analysis showed that the pulse with shorter rise time had higher amplitude of low frequencies and more high-frequency components, which resulted in a higher transmembrane potential of Staphylococcus aureus cells and higher effect on intracellular material.

引用

页码：1935 / 1941

页数：7

共 25 条

[1] Comparison of the effectiveness of biphase and monophase rectangular pulses for the inactivation of micro-organisms using pulsed electric fields [J].