Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins

被引：28

作者：

Yoon, Jae-Wook ^{[1
]}

Jung, Jae-Yeon ^{[1
]}

Chung, Hyun-Jung ^{[1
]}

Kim, Mi-Ryung ^{[2
]}

Kim, Chan-Wha ^{[2
]}

Lim, Seung-Taik ^{[1
]}

机构：

[1] Korea Univ, Grad Sch Life Sci & Biotechnol, Seoul 136701, South Korea

[2] Silla Univ, Dept Biofood Mat, Pusan 617736, South Korea

来源：

JOURNAL OF CEREAL SCIENCE | 2010年 / 52卷 / 02期

关键词：

Starch granule-associated protein (SGAP); SDS-PAGE; Starch noodle; Botanical origin; BOUND PROTEINS; SYNTHASE; MAIZE; RICE; EXPRESSION; ENZYMES; BARLEY;

D O I：

10.1016/j.jcs.2010.06.015

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Starch granule-associated proteins (SGAPs) were extracted from various starches and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. The SDS-PAGE results showed that granule-bound starch synthase (GBSS) of approximately 60 kDa was found in most starches, except waxy-type starches. Starches exhibited the presence of proteins specific to the botanical origin, including those of 22 kDa for maize; 160 and 98 kDa for potato; 140, 115, 90, and 80 kDa for wheat. These proteins could be detected from noodles prepared with the corresponding starches. The detection of the specific proteins by SDS-PAGE may be used to identify the origin of starch incorporated in various foods and industrial products. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：321 / 326

页数：6

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