Study of the Antioxidant and Anti-Inflammatory Properties of the Biological Extracts of Psophocarpus tetragonolobus Using Two Extraction Methods

被引：7

作者：

Bassal, Hussein ^{[1
,2
]}

Hijazi, Akram ^{[1
]}

Farhan, Hussein ^{[3
]}

Trabolsi, Christine ^{[1
]}

Ahmad, Bouchra Sayed ^{[1
]}

Khalil, Alia ^{[1
]}

Maresca, Marc ^{[4
]}

El Omar, Fawaz ^{[1
]}

机构：

[1] Lebanese Univ, Doctoral Sch Sci & Technol, Res Platform Environm Sci PRASE, POB 6573, Beirut, Lebanon

[2] Lebanese Univ, Fac Sci, Lab Canc Biol & Mol Immunol, POB 6573, Beirut, Lebanon

[3] Univ Baghdad, Coll Sci, Biotechnol Dept, Baghdad 10070, Iraq

[4] Aix Marseille Univ, iSm2, Cent Marseille, CNRS, F-13397 Marseille, France

来源：

MOLECULES | 2021年 / 26卷 / 15期

关键词：

winged bean; nitric oxide synthase; cyclooxygenase-2; ultrasound-assisted extraction; maceration; interleukin-6; ULTRASOUND-ASSISTED EXTRACTION; MEDICINAL-PLANTS; INFLAMMATION; EXPRESSION; CANCER; DC;

D O I：

10.3390/molecules26154435

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Psophocarpus tetragonolobus has long been used in traditional medicine and cuisine. In this study, Psophocarpus tetragonolobus extracts were isolated by maceration and ultrasound-assisted extraction and were evaluated for their antioxidant and anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The obtained results show that both extracts (maceration and ultrasound) were rich in bioactive molecules and exerted substantial antioxidant and anti-inflammatory effects. The P. tetragonolobus extracts' treatment in LPS-stimulated RAW264.7 macrophages resulted in a significant downregulation of the expressions of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and IL-1 beta mRNA. In addition, the P. tetragonolobus extracts' treatment attenuated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression. Our observations indicate that there is no significant difference between the two studied extracts of P. tetragonolobus in terms of biological properties (specifically, antioxidant and anti-inflammatory effects. Regardless of the extraction method, P. tetragonolobus could be used for treating diseases related to oxidative stress and inflammatory reactions.

引用

页数：12

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