Catalytic inactivation of human carbonic anhydrase I by a metallopeptide-sulfonamide conjugate is mediated by oxidation of active site residues

被引：32

作者：

Gokhale, Nikhil H. ^{[1
]}

Bradford, Seth ^{[1
]}

Cowan, J. A. ^{[1
]}

机构：

[1] Ohio State Univ, Evans Lab Chem, Columbus, OH 43210 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 08期

关键词：

D O I：

10.1021/ja0778038

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oxidation of active site residues (His and Trp), following catalytic inactivation of human carbonic anhydrase I by a copper-ATCUN conjugate of sulfanilimide, is evidenced by mass spectrometric analysis of tryptic and chymotryptic digest of the modified CA-I. Accordingly, residue oxidation rather than protein cleavage is the demonstrated mods of inactivation. An apparent second-order rate constant, k(2) similar to 7600 M(-1)min(-1), has been determined for catalytic inactivation of CA-I.

引用

页码：2388 / 2389

页数：2

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