DNA extraction and PCR methods for the detection of Listeria monocytogenes in cold-smoked salmon

被引：87
作者：
Simon, MC [1 ]
Grey, DI [1 ]
Cook, N [1 ]
机构：
[1] CSL, FOOD SCI LAB, ABERDEEN AB9 8DG, SCOTLAND
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 03期
关键词：
D O I：
10.1128/AEM.62.3.822-824.1996
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Protocols for the specific detection of listeria monocytogenes in cold-smoked salmon were developed. PCR was used as the method of detection. Inhibitors of PCR present in the food samples were removed by ether extraction or column purification, or their effect was overcome by the use of Tween 20 as an enhancer. These protocols are many times more rapid than conventional detection methodologies and also have the potential for automation.
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页码：822 / 824
页数：3
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