Different molecular sizes and chain conformations of water-soluble yeast β-glucan fractions and their interactions with receptor Dectin-1

被引：19
|
作者：
Zheng, Zhaomin [1 ,2 ]
Huang, Qilin [1 ]
Kang, Yu [3 ]
Liu, Yonggang [3 ]
Luo, Wei [4 ]
机构：
[1] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
[2] Hubei Univ Econ, Dept Cuisine & Nutr, Wuhan 430205, Peoples R China
[3] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
[4] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Changsha 410114, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2021年 / 273卷
基金：
中国国家自然科学基金;
关键词：
Water-soluble yeast beta-glucan; Molecular size; Chain conformation; Receptor Dectin-1; Interaction; ANTITUMOR-ACTIVITY; LIGHT-SCATTERING; SERUM-ALBUMIN; POLYSACCHARIDE; WEIGHT; FLUORESCENCE; BINDING; LIGAND; COMPLEXES; LENTINAN;
D O I：
10.1016/j.carbpol.2021.118568
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Although beta-glucan could bind to Dectin-1 to exert bioactivity, the influence of molecular size and chain conformation of beta-glucan on its interaction with Dectin-1 is still unclear. This work investigated the molecular sizes and chain conformations of five water-soluble yeast beta-glucan (WYG1-5) fractions as well as their interactions with Dectin-1 by fluorescence spectroscopy and microscale thermophoresis. Results revealed a spherical conformation for higher molecular weight WYG and a stiff chain conformation for smaller molecular weight WYG. The WYG and Dectin-1 interactions were in the order of WYG-2 > WYG-1 > WYG-3 > WYG-4 > WYG-5. The spherical WYG-2 exhibited the largest binding constant of 7.91 x 10(5) M-1 and the lowest dissociation constant of 22.1 nM to Dectin-1. Additionally, the underlying interaction mechanism showed that it may be easier for spherical WYG with longer side chains to interact with receptor Dectin-1.
引用
收藏
页数：13
相关论文
共 20 条
  • [1] Molecular dynamics modeling of different conformations of beta-glucan, molecular docking with dectin-1, and the effects on macrophages
    Huo, Rui
    Wuhanqimuge
    Zhang, Meili
    Sun, Minjun
    Miao, Ying
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2025, 293
  • [2] Molecular Basis of Interactions between β-Glucan and Dectin-1 and Their Application in Gene Delivery
    Takeda, Yoichi
    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, 2014, 26 (152) : 171 - 173
  • [3] The role of SIGNR1 and the β-glucan receptor (Dectin-1) in the nonopsonic recognition of yeast by specific macrophages
    Taylor, PR
    Brown, GD
    Herre, J
    Williams, DL
    Willment, JA
    Gordon, S
    JOURNAL OF IMMUNOLOGY, 2004, 172 (02): : 1157 - 1162
  • [4] Water-soluble yeast β-glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity
    Zheng, Zhaomin
    Huang, Qilin
    Ling, Chuqi
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 123 : 269 - 279
  • [5] FLOW-INDUCED MOLECULAR-INTERACTIONS AND CONFORMATIONS OF WATER-SOLUBLE POLYMERS
    KIM, OK
    CHOI, LS
    LONG, T
    YOON, T
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1987, 194 : 174 - PMSE
  • [6] Water-soluble polysaccharides from Pleurotus eryngii fruiting bodies, their activity and affinity for Toll-like receptor 2 and dectin-1
    Ellefsen, Christiane Faerestrand
    Wold, Christian Winther
    Wilkins, Alistair L.
    Rise, Frode
    Samuelsen, Anne Berit C.
    CARBOHYDRATE POLYMERS, 2021, 264
  • [7] Expression of the β-glucan receptor, dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions
    Reid, DM
    Montoya, M
    Taylor, PR
    Borrow, P
    Gordon, S
    Brown, GD
    Wong, SYC
    JOURNAL OF LEUKOCYTE BIOLOGY, 2004, 76 (01) : 86 - 94
  • [8] Hydrocarbons Depending on the Chain Length and Head Group Adopt Different Conformations within a Water-Soluble Nanocapsule: 1H NMR and Molecular Dynamics Studies
    Choudhury, Rajib
    Barman, Arghya
    Prabhakar, Rajeev
    Ramamurthy, V.
    JOURNAL OF PHYSICAL CHEMISTRY B, 2013, 117 (01): : 398 - 407
  • [9] Alveolar macrophage-mediated killing of Pneumocystis carinii f. sp muris involves molecular recognition by the dectin-1 β-glucan receptor
    Steele, C
    Marrero, L
    Swain, S
    Harmsen, AG
    Zheng, MQ
    Brown, GD
    Gordon, S
    Shellito, JE
    Kolls, JK
    JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 198 (11): : 1677 - 1688
  • [10] Mitogenic activity of particulate yeast beta-(1->3)-D-glucan and its water-soluble derivatives
    Sandula, J
    Machova, E
    Hribalova, V
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1995, 17 (06) : 323 - 326
12