Coexistence of two kinds of 6-phospho-beta-galactosidase in the cytosol of Lactobacillus gasseri JCM1031 - Purification and characterization of 6-phospho-beta-galactosidase II

被引：8

作者：

Suzuki, M

Saito, T

Itoh, T

机构：

[1] Laboratory of Animal Products Chemistry, Department of Applied Bio-Sciences, Faculty of Agriculture and Tohoku University, Sendai, 981, Aoba-ku

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 04期

关键词：

6-phospho-beta-galactosidase; Lactobacillus gasseri; purification;

D O I：

10.1271/bbb.60.708

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lactobacillus (L.) gasseri JCM1031 has 2 kinds of 6-phospho-beta-galactosidase (P-beta-gal) in the cytosol. P-beta-gal I was already isolated and characterized for our previous paper [Biosci, Biotech. Biochem., 60, 139-141 (1996)], Another, P-beta-gal II, was purified to homogeneity through several liquid chromatographic-steps for this paper. The molecular mass of the purified enzyme was estimated to be 58 kDa by SDS-PAGE. The K-m and V-max for ONPGal-6P were 0.76 mM and 86.0 mu mol/min/mg, respectively. Reducing reagents and Fe2+ ion activated the enzyme, but PCMB, Zn2+, and Hg2+ ions, and alkylation reagents strongly inhibited it. Twenty-five residues of the N-terminal amino acid sequence of the enzyme were identified. P-beta-gal II was different from P-P-gal I in several characteristics.

引用

页码：708 / 710

页数：3

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