Molecular identification and phylogenetic status of marine Bacillus associated with coral sediment, showing antibacterial effects against human pathogens

被引：6

作者：

Chatterjee, Shruti ^{[1
,2
]}

Haldar, Soumya ^{[2
]}

Asakura, Masahiro ^{[2
]}

Yamasaki, Shinji ^{[2
]}

Balasubramanian, Thangavel ^{[1
]}

机构：

[1] Ctr Adv Study Marine Biol, Porto Novo 608502, Tamil Nadu, India

[2] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Osaka 5998531, Japan

来源：

ANNALS OF MICROBIOLOGY | 2008年 / 58卷 / 02期

关键词：

antibacterial effects; Bacillus; coral sediments; gyraseB gene; phylogeny; 16S rRNA gene sequence;

D O I：

10.1007/BF03175335

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ninety-three strains were collected from coral reef sediments and artificial mangrove forest in south India and screened for antibacterial property by cross-streaking assay against 11 human pathogens. Five different fractions of three positive strains (GM4, GM5 and GM10) were prepared and culture filtrate (CF), ethyl acetate extracts (EA) and intermediate opaque layer (IOL) fractions showed antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enterica, Bacillus sp. and Proteus vulgaris. Genotyping using partial 16S rRNA gene and partial gyraseB sequencing revealed molecular identification (GM4-Bacillus cereus, GM5-Bacillus licheniformis and GM10-Bacillus subtilis), phylogenetic status and inter specific relationship among these three strains.

引用

页码：309 / 312

页数：4