Sequencing, phylogenetic and expression analysis of 16S rRNA gene in Rhizobium from chickpea

Chickpea, related to the family of Fabaceae, is growing worldwide due to its high importance. It is considered an alternative to meat due to the high amount of protein and vitamin contents. It is a significantly more manageable and healthier source of different kinds of proteins. Its medicinal importance cannot be neglected due to the presence of carbohydrates, fibers, polysaccharides, and oligosaccharides. Rhizobium bacteria have been found in chickpea roots' nodules to fix the nitrogen from the soil environment. A study of genetic diversity was performed to identify the best rhizobia strain, which improves the healthy amount of nitrogen. 16S rRNA gene was used for this purpose and this gene was also used for the phylogenetic analysis. Cloned sequenced analysis of 16S rRNA showed a high level of homology with others. Moreover, we identified and characterized eight other 16S rRNA from NCBI to analyze the multiple sequences and conserved motif analysis. Except it, by using appropriate tool, Sub-Cellular localization of all genes also predicted. To find the role of 16S rRNA genes in growth of chickpea, we performed expression analysis at different growth stage. This study will helpful for genetic and molecular aspect for further discussion.