Evaluation of microbial quality and yeast diversity in fresh-cut apple

被引：58

作者：

Graca, Ana ^{[1
,2
,3
]}

Santo, David ^{[1
]}

Esteves, Eduardo ^{[4
,5
]}

Nunes, Carla ^{[6
]}

Abadias, Maribel ^{[7
]}

Quintas, Celia ^{[1
]}

机构：

[1] Univ Algarve, Inst Super Engn, P-8005139 Faro, Portugal

[2] Univ Algarve, Fac Ciencias & Tecnol, P-7006554 Evora, Portugal

[3] Univ Evora, ICAAM, P-7006554 Evora, Portugal

[4] Univ Algarve, Inst Super Engn, P-8005139 Faro, Portugal

[5] Univ Algarve, Ctr Ciencias Mar Algarve CCMAR, CIMAR Lab Associado, P-8005139 Faro, Portugal

[6] Univ Algarve, Ctr Empresarial Gambelas, P-8005139 Faro, Portugal

[7] IRTA, XaRTA Postharvest, Lleida 25003, Catalonia, Spain

来源：

FOOD MICROBIOLOGY | 2015年 / 51卷

关键词：

Fresh-cut apple; Microbial quality; Coliforms; Staphylococci; Spoilage; Yeasts; RIBOSOMAL-RNA GENE; ESCHERICHIA-COLI; CANDIDA-SAKE; MICROBIOLOGICAL QUALITY; BLUE MOLD; BIOLOGICAL-CONTROL; BIOCONTROL AGENT; IDENTIFICATION; STRAIN; FRUITS;

D O I：

10.1016/j.fm.2015.06.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present work's aim was to study the microbial quality of minimally processed apples commercialized in Portugal. Sixty eight samples of fresh-cut apple were analyzed before their best-before date in 2011 and 2012 for aerobic mesophilic and psychrotrophic microorganisms, total coliforms, lactic-acid bacteria (LAB), coagulase-positive staphylococci and fungi. The parameters of food safety studied were Cronobacter sakazakii, Salmonella spp. and Listeria sp. Samples were analyzed according to standard methodologies and using Chromocult Agar for coliforms and Escherichia coli. The yeasts were identified by restriction analysis of the ITS-5.8S rDNA-region and 26S rDNA partial sequencing. The mesophilic and psychrotrophic microorganisms ranged from 3.3 to 8.9 and from 4.9 to 8.4 log CFU/g, respectively. Coliforms were detected in all the samples and staphylococci in 5.8% of them. LAB numbers varied from 2.8 to 8.7 and fungi (yeast and molds) from 3.6 to 7.1 log CFU/g. The most common yeasts were Candida sake and Pichia fermentans followed by Hanseniaspora spp., Candida spp., Meyerozyma guilliermondii, Metschnikowia pulcherrima, Cryptococcus spp. and the psychrotrophic Cystofilobasidium infirmominiatum. Foodborne bacteria and opportunistic pathogenic yeasts were not detected in the apples studied. The results obtained respected the European Commission regulation regarding criteria of food hygiene and safety. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：179 / 185

页数：7

共 51 条

[1] Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments [J].

Abadias, M. ;

Usall, J. ;

Anguera, M. ;

Solson, C. ;

Vinas, I. .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2008, 123 (1-2) :121-129

[2] Microbial quality of commercial 'Golden Delicious' apples throughout production and shelf-life in Lleida (Catalonia, Spain) [J].

Abadias, M ;

Cañamás, TP ;

Asensio, A ;

Anguera, M ;

Viñas, I .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2006, 108 (03) :404-409

[3] Fate of Escherichia coli in apple and reduction of its growth using the postharvest biocontrol agent Candida sake CPA-1 [J].

Abadias, Maribel ;

Usall, Josep ;

Alegre, Isabel ;

Torres, Rosario ;

Vinas, Inmaculada .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2009, 89 (09) :1526-1533

[4] Microbiological and physicochemical quality of fresh-cut apple enriched with the probiotic strain Lactobacillus rhamnosus GG [J].

Alegre, Isabel ;

Vinas, Inmaculada ;

Usall, Josep ;

Anguera, Marina ;

Abadias, Maribel .

FOOD MICROBIOLOGY, 2011, 28 (01) :59-66

[5] Fate of Escherichia coli O157:H7, Salmonella and Listeria innocua on minimally-processed peaches under different storage conditions [J].

Alegre, Isabel ;

Abadias, Maribel ;

Anguera, Marina ;

Usall, Josep ;

Vinas, Inmaculada .

FOOD MICROBIOLOGY, 2010, 27 (07) :862-868

[6] Factors affecting growth of foodborne pathogens on minimally processed apples [J].

Alegre, Isabel ;

Abadias, Maribel ;

Anguera, Marina ;

Oliveira, Marcia ;

Vinas, Inmaculada .

FOOD MICROBIOLOGY, 2010, 27 (01) :70-76

[7]

[Anonymous], 1989, Cold Spring Harbor

[8] Fresh fruit and vegetables as vehicles for the transmission of human pathogens [J].

Berger, Cedric N. ;

Sodha, Samir V. ;

Shaw, Robert K. ;

Griffin, Patricia M. ;

Pink, David ;

Hand, Paul ;

Frankel, Gad .

ENVIRONMENTAL MICROBIOLOGY, 2010, 12 (09) :2385-2397

[9] Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables [J].

Beuchat, LR .

MICROBES AND INFECTION, 2002, 4 (04) :413-423

[10] Isolation of catalase-negative Listeria monocytogenes strains from listeriosis patients and their rapid identification by anti-p60 antibodies and/or PCR [J].

Bubert, A ;

Riebe, J ;

Schnitzler, N ;

Schonberg, A ;

Goebel, W ;

Schubert, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (01) :179-183

← 1 2 3 4 5 6 →