Genome-Scale Metabolic Network Reconstruction and In Silico Analysis of Hexanoic acid Producing Megasphaera elsdenii
被引:18
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作者:
Lee, Na-Rae
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Konkuk Univ, Dept Biosci & Biotechnol, Seoul 05029, South Korea
Ewha Womans Univ, Dept Food Sci & Engn, Seoul 03760, South KoreaKonkuk Univ, Dept Biosci & Biotechnol, Seoul 05029, South Korea
Lee, Na-Rae
[1
,2
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Lee, Choong Hwan
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机构:
Konkuk Univ, Dept Biosci & Biotechnol, Seoul 05029, South KoreaKonkuk Univ, Dept Biosci & Biotechnol, Seoul 05029, South Korea
Lee, Choong Hwan
[1
]
Lee, Dong-Yup
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Sungkyunkwan Univ, Sch Chem Engn, 2066 Seobu Ro, Suwon 16419, Gyeonggi Do, South KoreaKonkuk Univ, Dept Biosci & Biotechnol, Seoul 05029, South Korea
Lee, Dong-Yup
[3
]
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机构:
Park, Jin-Byung
[2
]
机构:
[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 05029, South Korea
[2] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 03760, South Korea
[3] Sungkyunkwan Univ, Sch Chem Engn, 2066 Seobu Ro, Suwon 16419, Gyeonggi Do, South Korea
Hexanoic acid and its derivatives have been recently recognized as value-added materials and can be synthesized by several microbes. Of them, Megasphaera elsdenii has been considered as an interesting hexanoic acid producer because of its capability to utilize a variety of carbons sources. However, the cellular metabolism and physiology of M. elsdenii still remain uncharacterized. Therefore, in order to better understand hexanoic acid synthetic metabolism in M. elsdenii, we newly reconstructed its genome-scale metabolic model, iME375, which accounts for 375 genes, 521 reactions, and 443 metabolites. A constraint-based analysis was then employed to evaluate cell growth under various conditions. Subsequently, a flux ratio analysis was conducted to understand the mechanism of bifurcated hexanoic acid synthetic pathways, including the typical fatty acid synthetic pathway via acetyl-CoA and the TCA cycle in a counterclockwise direction through succinate. The resultant metabolic states showed that the highest hexanoic acid production could be achieved when the balanced fractional contribution via acetyl-CoA and succinate in reductive TCA cycle was formed in various cell growth rates. The highest hexanoic acid production was maintained in the most perturbed flux ratio, as phosphoenolpyruvate carboxykinase (pck) enables the bifurcated pathway to form consistent fluxes. Finally, organic acid consuming simulations suggested that succinate can increase both biomass formation and hexanoic acid production.
机构:
Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USAUniv Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Milne, Caroline B.
Eddy, James A.
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机构:
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA
Univ Illinois, Dept Bioengn, Urbana, IL 61801 USAUniv Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Eddy, James A.
Raju, Ravali
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Univ Minnesota, Dept Chem Engn & Mat Sci, Minneapolis, MN 55455 USAUniv Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Raju, Ravali
Ardekani, Soroush
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机构:Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Ardekani, Soroush
Kim, Pan-Jun
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Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USAUniv Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Kim, Pan-Jun
Senger, Ryan S.
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机构:
Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA USAUniv Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Senger, Ryan S.
Jin, Yong-Su
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机构:
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA
Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USAUniv Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Jin, Yong-Su
Blaschek, Hans P.
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机构:
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA
Univ Illinois, Ctr Adv BioEnergy Res, Urbana, IL 61801 USA
Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USAUniv Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Blaschek, Hans P.
Price, Nathan D.
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机构:
Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA
Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA
Inst Syst Biol, Seattle, WA 98109 USAUniv Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA