MiR-377 suppresses cell proliferation and metastasis in gastric cancer via repressing the expression of VEGFA

被引:4
作者
Wang, C. -Q. [1 ]
Chen, L. [2 ,3 ]
Dong, C. -L. [4 ]
Song, Y. [1 ]
Shen, Z. -P. [5 ]
Shen, W. -M. [1 ]
Wu, X. -D. [1 ]
机构
[1] Yancheng City 1 Peoples Hosp, Dept Gastroenterol, Yancheng, Peoples R China
[2] Nanjing Med Univ, Affiliated Hosp 2, Dept Digest Endoscopy, Nanjing, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Affiliated Hosp 2, Med Ctr Digest Dis, Nanjing, Jiangsu, Peoples R China
[4] Yancheng City 1 Peoples Hosp, Dept Emergency, Yancheng, Peoples R China
[5] Yancheng City 1 Peoples Hosp, Dept Digest Endoscopy, Yancheng, Peoples R China
关键词
miR-377; Gastric cancer; Proliferation; Metastasis; VEGFA; TUMOR-SUPPRESSOR; DOWN-REGULATION; GROWTH; CARCINOMA; PROMOTES; INVASION; PATHWAY; ANGIOGENESIS; PATHOGENESIS; CONTRIBUTES;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
OBJECTIVE: In recent years, microRNAs have been identified to participate in tumor genesis and progression of different tumors including gastric cancer. However, the role of miR-377 played in gastric cancer (GC), and its mechanisms have not been demonstrated. PATIENTS AND METHODS: We detected miR-377 expression level in 86 GC and adjacent normal tissue samples by quantitative reverse transcription PCR (qRT-PCR) as well as in GC cell lines. The relationship between miR-377 and clinical pathological features was analyzed. Using miR-377 mimics and inhibitors, we interfered with miR-377 level and employed several functional experiments to study the miR-377 effects on cell proliferation, migration, and invasion. Western blot assay and dual-luciferase assay were used to verify the target of miR-377. RESULTS: miR-377 expressed significantly lower in GC tissues and cell lines compared to normal tissues and GES-1 cells. Overexpression of miR-377 inhibited cell growth, migration and invasion, while downregulation miR-377 obviously promoted cell growth and metastasis. Furthermore, vascular endothelial growth factor A (VEGFA) was confirmed as a direct target of miR-377 and reversed the influence of mir-377 over-expression. CONCLUSIONS: miR-377 expressed lower in GC and suppressed cell proliferation, migration and invasion partly via repressing the VEGFA expression, which could provide a potential target for GC diagnosis and therapy.
引用
收藏
页码:5101 / 5111
页数:11
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