Molecular characterization of an Arabidopsis gene encoding hydroperoxide lyase, a cytochrome P-450 that is wound inducible

被引:107
作者
Bate, NJ
Sivasankar, S
Moxon, C
Riley, JMC
Thompson, JE
Rothstein, SJ
机构
[1] Univ Guelph, Dept Mol Biol & Genet, Guelph, ON N1G 2W1, Canada
[2] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada
关键词
D O I
10.1104/pp.117.4.1393
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Hydroperoxide lyase (HPL) cleaves lipid hydroperoxides to produce volatile flavor molecules and also potential signal molecules. We have characterized a gene from Arabidopsis that is homologous to a recently cloned HPL from green pepper (Capsicum annuum). The deduced protein sequence indicates that this gene encodes a cytochrome P-450 with a structure similar to that of allene oxide synthase. The gene was cloned into an expression vector and expressed in Escherichia coli to demonstrate HPL activity. Significant HPL activity was evident when 13S-hydroperoxy-9(Z), 11(E), 15(Z)-octadecatrienoic acid was used as the substrate, whereas activity with 13S-hydroperoxy-9(Z),11(E)-octadecadienoic acid was approximately 10-fold lower. Analysis of headspace volatiles by gas chromatography-mass spectrometry, after addition of the substrate to E. coli extracts expressing the protein, confirmed enzyme-activity data, since cis-3-hexenal was produced by the enzymatic activity of the encoded protein, whereas hexanal production was limited. Molecular characterization of this gene indicates that it is expressed at high levels in floral tissue and is wound inducible but, unlike allene oxide synthase, it is not induced by treatment with methyl jasmonate.
引用
收藏
页码:1393 / 1400
页数:8
相关论文
共 33 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]  
ANDERSON JM, 1989, 2ND MESSENGERS PLANT, V6, P181
[3]   Identifying natural volatile compounds that control gray mold (Botrytis cinerea) during postharvest storage of strawberry, blackberry, and grape [J].
Archbold, DD ;
HamiltonKemp, TR ;
Barth, MM ;
Langlois, BE .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (10) :4032-4037
[4]  
Ausubel F. M., 1994, CURRENT PROTOCOLS MO
[5]   EFFECT OF VOLATILE METHYL JASMONATE ON THE OXYLIPIN PATHWAY IN TOBACCO, CUCUMBER, AND ARABIDOPSIS [J].
AVDIUSHKO, S ;
CROFT, KPC ;
BROWN, GC ;
JACKSON, DM ;
HAMILTONKEMP, TR ;
HILDEBRAND, D .
PLANT PHYSIOLOGY, 1995, 109 (04) :1227-1230
[6]   COI1-DEPENDENT EXPRESSION OF AN ARABIDOPSIS VEGETATIVE STORAGE PROTEIN IN FLOWERS AND SILIQUES AND IN RESPONSE TO CORONATINE OR METHYL JASMONATE [J].
BENEDETTI, CE ;
XIE, DX ;
TURNER, JG .
PLANT PHYSIOLOGY, 1995, 109 (02) :567-572
[7]   Envelope membranes from spinach chloroplasts are a site of metabolism of fatty acid hydroperoxides [J].
Blee, E ;
Joyard, J .
PLANT PHYSIOLOGY, 1996, 110 (02) :445-454
[8]   METHYL JASMONATE INDUCES PAPAIN INHIBITOR(S) IN TOMATO LEAVES [J].
BOLTER, CJ .
PLANT PHYSIOLOGY, 1993, 103 (04) :1347-1353
[9]   A NUCLEAR GENE ENCODING THE BETA-SUBUNIT OF THE MITOCHONDRIAL ATP-SYNTHASE IN NICOTIANA-PLUMBAGINIFOLIA [J].
BOUTRY, M ;
CHUA, NH .
EMBO JOURNAL, 1985, 4 (09) :2159-2165
[10]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3