Building and Searching Tandem Mass Spectral Libraries for Peptide Identification

被引:50
作者
Lam, Henry [1 ]
机构
[1] Hong Kong Univ Sci & Technol, Dept Chem & Biomol Engn, Hong Kong, Hong Kong, Peoples R China
关键词
INDUCED DISSOCIATION SPECTRA; LARGE-SCALE PROTEOMICS; PROTEIN IDENTIFICATION; POSTTRANSLATIONAL MODIFICATIONS; COMPUTATIONAL ANALYSIS; MS/MS SPECTRA; SPECTROMETRY DATA; YEAST PROTEOME; DATA SETS; DATABASE;
D O I
10.1074/mcp.R111.008565
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Spectral library searching is an emerging approach in peptide identifications from tandem mass spectra, a critical step in proteomic data analysis. Conceptually, the premise of this approach is that the tandem MS fragmentation pattern of a peptide under some fixed conditions is a reproducible fingerprint of that peptide, such that unknown spectra acquired under the same conditions can be identified by spectral matching. In actual practice, a spectral library is first meticulously compiled from a large collection of previously observed and identified tandem MS spectra, usually obtained from shotgun proteomics experiments of complex mixtures. Then, a query spectrum is then identified by spectral matching using recently developed spectral search engines. This review discusses the basic principles of the two pillars of this approach: spectral library construction, and spectral library searching. An overview of the software tools available for these two tasks, as well as a high-level description of the underlying algorithms, will be given. Finally, several new methods that utilize spectral libraries for peptide identification in ways other than straightforward spectral matching will also be described. Molecular & Cellular Proteomics 10: 10.1074/mcp.R111.008565, 1-10, 2011.
引用
收藏
页数:10
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