MICRONUCLEUS ASSAY AS A BIOMARKER FOR CHROMOSOME MALSEGREGATION IN YOUNG MOTHERS WITH DOWN SYNDROME CHILDREN

被引：0
作者：
Hady, S. Abdel [1 ]
Afifi, H. H. [2 ]
Ghany, E. A. Abdel [1 ]
Taher, M. B. [2 ]
Eid, M. M. [3 ]
机构：
[1] Cairo Univ, Fac Med, Dept Pediat, Cairo, Egypt
[2] Natl Res Ctr, Dept Clin Genet, Giza, Egypt
[3] Natl Res Ctr, Human Cytogenet Dept, Giza, Egypt
来源：
GENETIC COUNSELING | 2015年 / 26卷 / 01期
关键词：
Young mothers of Down syndrome; Micronucleus assay; FISH; Malsegregation; SUSCEPTIBILITY; LYMPHOCYTES; ANEUPLOIDY; DAMAGE;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Micronucleus assay as a biomarker for chromosome malsegregation in young mothers with Down syndrome children: The aim of the present study is to test the susceptibility of chromosome 21 malsegregation in young mothers of Down syndrome children using combined micronucleus (MN) assay and FISH analysis. The present study included 62 Egyptian young mothers (age <30 y) who were divided into 22 mothers of DS offspring and 40 age matched controls. All subjects were subjected to chromosomal analysis, micronucleus assay, and FISH analysis. High statistical significant difference was found between mothers of Down syndrome (MDS) and the controls in the MN percentage (P=0.034). Also there was high statistical significant difference between MDS and the controls in the percentage of positive malsegregation (P=0.0001). The specificity of combined MN% with FISH was 90%, while the sensitivity was 63.6%. Combined MN-FISH test is highly specific but moderately sensitive in assessing the risk of having children with DS in young mothers.
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页码：13 / 19
页数：7
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