Diagnostic Performance of Filter Paper Lesion Impression PCR for Secondarily Infected Ulcers and Nonulcerative Lesions Caused by Cutaneous Leishmaniasis

被引：17

作者：

Boggild, Andrea K. ^{[6
]}

Ramos, Ana Pilar ^{[1
]}

Valencia, Braulio Mark ^{[1
]}

Veland, Nicolas ^{[1
,2
]}

Calderon, Flor ^{[1
]}

Arevalo, Jorge ^{[1
,2
]}

Low, Donald E. ^{[3
,4
]}

Llanos-Cuentas, Alejandro ^{[1
,5
]}

机构：

[1] Univ Peruana Cayetano Heredia, Inst Med Trop Alexander von Humboldt, Lima, Peru

[2] Univ Peruana Cayetano Heredia, Fac Ciencias, Dept Bioquim & Biol Mol & Farmacol, Lima, Peru

[3] Ontario Agcy Hlth Protect & Promot, Labs Branch, Etobicoke, ON, Canada

[4] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[5] Hosp Nacl Cayetano Heredia, Lima, Peru

[6] UHN Toronto Gen Hosp, Trop Dis Unit, Div Infect Dis, Toronto, ON, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 03期

关键词：

MOLECULAR DIAGNOSIS; MICROCULTURE METHOD; IDENTIFICATION; PARASITES; ASSAY; PERU;

D O I：

10.1128/JCM.02457-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We compared traditional cutaneous leishmaniasis diagnostic methods to filter paper lesion impression (FPLI) PCR for secondarily infected ulcers and nonulcerative lesions. The sensitivity and specificity of FPLI PCR for secondarily infected lesions (n = 8) were 100%. In primarily nonulcerative lesions (n = 15), the sensitivity of FPLI PCR was inferior to that of pooled-invasive-specimen PCR (72.7% versus 100%) (P = 0.10). FPLI PCR is sensitive, specific, and unlike invasive procedures, can be used in secondarily infected ulcers. Invasive specimen collection is superior in nonulcerative lesions.

引用

页码：1097 / 1100

页数：4

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