Protein adsorption at a gold electrode studied by in situ scanning tunnelling microscopy

被引：73
作者：
Davis, JJ
Halliwell, CM
Hill, HAO [1 ]
Canters, GW
van Amsterdam, MC
Verbeet, MP
机构：
[1] Univ Oxford, Inorgan Chem Lab, Oxford OX1 3QR, England
[2] Leiden Univ, Dept Chem, Gorlaeus Lab, NL-2333 CC Leiden, Netherlands
来源：
NEW JOURNAL OF CHEMISTRY | 1998年 / 22卷 / 10期
关键词：
D O I：
10.1039/a803384f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The in situ adsorption, under physiological conditions, of azurin molecules at a gold electrode surface has been monitored at the molecular level by scanning tunnelling microscopy. The introduction of free cysteine residues into the surface of the redox protein by structurally conservative mutagenesis allows the immobilisation to be controlled in a manner in which the protein electrochemical activity is retained.
引用
收藏
页码：1119 / 1123
页数：5
相关论文
共 45 条
[11]   Dynamics of Pseudomonas aeruginosa azurin and its Cys3Ser mutant at single-crystal gold surfaces investigated by cyclic voltammetry and atomic force microscopy [J].
Friis, EP ;
Andersen, JET ;
Madsen, LL ;
Bonander, N ;
Moller, P ;
Ulstrup, J .
ELECTROCHIMICA ACTA, 1997, 42 (19) :2889-2897
[12]   IMAGING OF UNCOATED PURPLE MEMBRANE BY SCANNING TUNNELING MICROSCOPY [J].
GUCKENBERGER, R ;
HACKER, B ;
HARTMANN, T ;
SCHEYBANI, T ;
WANG, Z ;
WIEGRABE, W ;
BAUMEISTER, W .
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 1991, 9 (02) :1227-1230
[13]   ATOMIC FORCE MICROSCOPY AND DISSECTION OF GAP-JUNCTIONS [J].
HOH, JH ;
LAL, R ;
JOHN, SA ;
REVEL, JP ;
ARNSDORF, MF .
SCIENCE, 1991, 253 (5026) :1405-1408
[14]   OPTICAL DETERMINATION OF SURFACE-DENSITY IN ORIENTED METALLOPROTEIN NANOSTRUCTURES [J].
HONG, HG ;
BOHN, PW ;
SLIGAR, SG .
ANALYTICAL CHEMISTRY, 1993, 65 (11) :1635-1638
[15]   CYSTEINE-SPECIFIC SURFACE TETHERING OF GENETICALLY-ENGINEERED CYTOCHROMES FOR FABRICATION OF METALLOPROTEIN NANOSTRUCTURES [J].
HONG, HG ;
JIANG, M ;
SLIGAR, SG ;
BOHN, PW .
LANGMUIR, 1994, 10 (01) :153-158
[16]   REFLECTION ELECTRON-MICROSCOPY (REM) OF FCC METALS [J].
HSU, T ;
COWLEY, JM .
ULTRAMICROSCOPY, 1983, 11 (04) :239-250
[17]   ADDITION OF SULFHYDRYL-GROUPS TO ESCHERICHIA-COLI RIBOSOMES BY PROTEIN MODIFICATION WITH 2-IMINOTHIOLANE (METHYL 4-MERCAPTOBUTYRIMIDATE) [J].
JUE, R ;
LAMBERT, JM ;
PIERCE, LR ;
TRAUT, RR .
BIOCHEMISTRY, 1978, 17 (25) :5399-5406
[18]   KINETIC-MODEL FOR SERUM-ALBUMIN ADSORPTION - EXPERIMENTAL-VERIFICATION [J].
KURRAT, R ;
RAMSDEN, JJ ;
PRENOSIL, JE .
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1994, 90 (04) :587-590
[19]   MANIPULATION OF PROTEINS ON MICA BY ATOMIC FORCE MICROSCOPY [J].
LEA, AS ;
PUNGOR, A ;
HLADY, V ;
ANDRADE, JD ;
HERRON, JN ;
VOSS, EW .
LANGMUIR, 1992, 8 (01) :68-73
[20]   STUDIES OF COVALENTLY IMMOBILIZED PROTEIN MOLECULES BY SCANNING-TUNNELING-MICROSCOPY - THE ROLE OF WATER IN IMAGE-CONTRAST FORMATION [J].
LEGGETT, GJ ;
DAVIES, MC ;
JACKSON, DE ;
ROBERTS, CJ ;
TENDLER, SJB ;
WILLIAMS, PM .
JOURNAL OF PHYSICAL CHEMISTRY, 1993, 97 (35) :8852-8854
12345