Protein adsorption at a gold electrode studied by in situ scanning tunnelling microscopy

被引：73
作者：
Davis, JJ
Halliwell, CM
Hill, HAO [1 ]
Canters, GW
van Amsterdam, MC
Verbeet, MP
机构：
[1] Univ Oxford, Inorgan Chem Lab, Oxford OX1 3QR, England
[2] Leiden Univ, Dept Chem, Gorlaeus Lab, NL-2333 CC Leiden, Netherlands
来源：
NEW JOURNAL OF CHEMISTRY | 1998年 / 22卷 / 10期
关键词：
D O I：
10.1039/a803384f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The in situ adsorption, under physiological conditions, of azurin molecules at a gold electrode surface has been monitored at the molecular level by scanning tunnelling microscopy. The introduction of free cysteine residues into the surface of the redox protein by structurally conservative mutagenesis allows the immobilisation to be controlled in a manner in which the protein electrochemical activity is retained.
引用
收藏
页码：1119 / 1123
页数：5
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